文献类型：期刊文献

英文题名：Colorimetric and fluorescent determination of sulfide and sulfite with kinetic discrimination

作者：Pei, Xiaoliang[1];Tian, Haiyu[1];Zhang, Weibing[1];Brouwer, Albert M.[2];Qian, Junhong[1]

机构：[1]E China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]Univ Amsterdam, Fac Sci, Vant Hoff Inst Mol Sci, NL-1090 GD Amsterdam, Netherlands

年份：2014

卷号：139

期号：20

起止页码：5290

外文期刊名：ANALYST

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000342393900033)】；

基金：This work was financially supported by National 973 Program (No. 2011CB910403), NSFC (21235005) and Science and Technology Commission of Shanghai Municipality (no. 12JG0500200).

语种：英文

摘要：Two fluorescent probes, m-PSP and p-PSP, for sulfite and/or sulfide were constructed by connecting a pyridinium ion to a coumarin fluorophore through an alpha,beta-unsaturated ketone. The presence of the pyridinium salt promoted the nucleophilic addition of sulfite and sulfide to the alpha,beta-unsaturated ketone, which could be visualized by dramatic changes in the solution's color and fluorescence intensity. Both probes exhibit good selectivity (the selectivity coefficients toward major interferences are less than 0.07) and high sensitivity for sulfite and sulfide over biothiols and other potential analytes. The detection limits of m-PSP for the analysis of sulfite and sulfide are calculated to 8.5 x 10(-7) M and 2.7 x 10(-7) M, respectively. Living cell imaging results indicate that both probes can be applied in biological systems.