文献类型：期刊文献

英文题名：Preparation of a novel light-sensitive copolymer and its application in recycling aqueous two-phase systems

作者：Wang, Wei[1];Wan, Junfen[1];Ning, Biao[1];Xia, Jinan[2];Cao, Xuejun[1]

机构：[1]E China Univ Sci & Technol, Dept Biochem Engn, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Donghua Univ, Dept Appl Phys, Shanghai 200051, Peoples R China

年份：2008

卷号：1205

期号：1-2

起止页码：171

外文期刊名：JOURNAL OF CHROMATOGRAPHY A

收录：;EI(收录号：20083711528772);WOS:【SCI-EXPANDED(收录号:WOS:000259884700021)】；

基金：This project was supported by Natural Scientific Foundation of China (20474016) and Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars.

语种：英文

外文关键词：aqueous two-phase systems; light-sensitive copolymer; pH-sensitive copolymer; chlorophyllin sodium copper

摘要：Aqueous two-phase systems (ATPSs) are potential bioseparation techniques in industry. However, a key problem is that aqueous two-phase systems could not be effectively recycled to result in high cost and environment pollution. Recently, how to prepare recycling copolymers forming aqueous two-phase systems is focused on in the area. In this study, a light-sensitive copolymer (P-NNC) was synthesized by using N-isopropylacrylamide (NIPA), N-vinyl-2-pyrrolidone (NVP), chlorophyllin sodium copper salt (CHL) as monomers. The copolymer PNNC can form ATPSs with another novel pH-sensitive copolymer (PADB) which was synthesized by co-worker in our laboratory. Over 98% of the PNNC copolymer could be recovered by using laser radiation at 488 nm. The copolymer PADB could be recovered by adjusting the isoelectric point (pI) to 4.1, with a recovery of 97%. Bovine serum albumin (BSA) and Tyr were partitioned in the P-NNC-P-ADB aqueous two-phase systems to examine the systems. It was found that partition coefficient of BSA and L-Tyr could reach 4.1 and 0.12 in the systems, respectively. (C) 2008 Elsevier B.V. All rights reserved.