文献类型：期刊文献

英文题名：Development of a fed-batch cultivation for antibody-producing cells based on combined feeding strategy of glucose and galactose

作者：Sun, Ya-ting[1];Zhao, Liang[1];Ye, Zhaoyang[1];Fan, Li[1];Liu, Xu-ping[1];Tan, Wen-Song[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Biareactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：81

起止页码：126

外文期刊名：BIOCHEMICAL ENGINEERING JOURNAL

收录：;EI(收录号：20134616974713);WOS:【SCI-EXPANDED(收录号:WOS:000329772500016)】；

基金：This work was supported by the National Natural Science Foundation of China (Nos. 21106045, 21206040), the National High Technology Research and Development Program of China (863 Program) (No. 2012AA02A303), the National Science and Technology Major Project (No. 2013ZX10004003-003-003), Shanghai Training Program for College Young Teacher (No. hlg11011).

语种：英文

外文关键词：CHO cells; Fed-batch culture; Antibody production; Lactate metabolism; Galactose; Process development

摘要：In order to achieve enhanced cell mass and productivity with less lactate accumulation, a fed-batch culture based on a combined feeding strategy of glucose and galactose was developed. Cell performance was first examined with feeding of galactose alone. While cell growth was improved compared with glucose-feeding culture, cell maintenance was inefficient with rapid lactate depletion and considerable ammonium accumulation. Subsequently, to improve cell maintenance, a combined feeding strategy of glucose and galactose was proposed focusing on optimizing the ratio of glucose to galactose and feeding time. In addition, the compositions of amino acids and vitamins in feeding medium were refined for balanced supply of nutrients. With the combined feeding strategy, the metabolic shift of lactate from production to consumption occurred, but not accompanied by rapid lactate depletion and ammonium production. Furthermore, energy metabolism was more efficient and better utilization of carbon sources was achieved. Compared with the glucose-feeding culture in bioreactor, maximum lactate concentration was reduced by 55%; IVCC and the specific production rate of antibody were increased by 45% and 143%, respectively. (C) 2013 Elsevier B.V. All rights reserved.