文献类型：期刊文献

英文题名：Optimisation of enzymatic synthesis of cefaclor with in situ product removal and continuous acyl donor feeding

作者：Zhang, Yewang; Wei, Dongzhi; Li, Dengchao; Liu, Shulai; Song, Qingxun

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, New World Inst Biotechnol, Shanghai 200237, Peoples R China

年份：2007

卷号：25

期号：1

起止页码：59

外文期刊名：BIOCATALYSIS AND BIOTRANSFORMATION

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000245108200009)】；

语种：英文

外文关键词：cefaclor; continuous acyl donor feeding; enzymatic synthesis; in situ product removal; penicillin acylase

摘要：Enzymatic synthesis of cefaclor by penicillin acylase (PA) was carried out under kinetic control with in situ product removal (ISPR). We present a continuous acyl donor feeding strategy for enzymatic reactions. Using this strategy, the conversion of the antibiotic nucleus was improved from 65 to 91%, and the hydrolysis of phenylglycine methyl ester ( PGME) was decreased. Side product ( phenylglycine) production was less than half of that in the control batch. The ratio of synthesis to hydrolysis (S/H) in the process was kept stable for longer and at a higher level than in the control. This is a practical method for enzymatic synthesis of cefaclor.