文献类型：期刊文献

英文题名：The characteristics of TAG and EPA accumulation in Nannochloropsis oceanica IMET1 under different nitrogen supply regimes

作者：Meng, Yingying[1,2];Jiang, Junpeng[1,3];Wang, Haitao[1,3];Cao, Xupeng[1];Xue, Song[1];Yang, Qing[2];Wang, Weiliang[4]

机构：[1]Chinese Acad Sci, Dalian Inst Chem Phys, Marine Bioprod Engn Grp, Dalian 116023, Peoples R China;[2]Dalian Univ Technol, Sch Life Sci & Biotechnol, Dalian 116024, Peoples R China;[3]Univ Chinese Acad Sci, Beijing 100049, Peoples R China;[4]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2015

卷号：179

起止页码：483

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20150300434715);WOS:【SCI-EXPANDED(收录号:WOS:000348041600064)】；

基金：This work was supported by National Key Basic Research Program of China "973 Program" (2011CB200903), National High Technology Research and Development Program "863" (2012AA052101 and 2014AA022004), Liaoning Provincial Natural Science Foundation (2012010263), the Hundred Talent Program of the Chinese Academy of Sciences (No. A1097) and Knowledge Innovation Programs of Dalian Institute of Chemical Physics, CAS (K2010A3).

语种：英文

外文关键词：Nannochloropsis oceanica IMET1; TAG; EPA; Nitrogen limitation; Fatty acid composition

摘要：The strategy of nitrogen limitation has been widely applied to enhance lipid production in microalgae. The changes of cellular composition, and the characteristics of triacylglycerol (TAG) and eicosapentaenoic acid (EPA) accumulation in Nannochloropsis oceanica IMET1 were investigated. The results revealed that after nitrogen limitation TAG rather than carbohydrate was the dominant carbon sink in N. oceanica IMET1. Different nitrogen supplementation strategies were applied in order to achieve high TAG and EPA productivity, respectively. Limited nitrogen was supplied to improve TAG production, and a maximum productivity of 29.44 mg L-1 d(-1) was obtained, which was a 6.74-fold increase compared to nitrogen-depleted cultivation. The highest EPA productivity of 7.66 mg L-1 d(-1) was achieved under nitrogenreplete cultivation, which is different from the condition for TAG maximum productivity because the EPA is in glycolipids and phospholipids mainly. The fatty acid composition analysis identified the source of acyl group in TAG accumulation. (C) 2014 Elsevier Ltd. All rights reserved.