文献类型：期刊文献

英文题名：Remote light-controlled intracellular target recognition by photochromic fluorescent glycoprobes

作者：Zhang, Junji[1,2];Fu, Youxin[1,2];Han, Hai-Hao[1,2,3];Zang, Yi[3];Li, Jia[3];He, Xiao-Peng[1,2];Feringa, Ben L.[4,5];Tian, He[1,2]

机构：[1]East China Univ Sci Technol, Sch Chem & Mol Engn, Key Lab Adv Mat, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci Technol, Sch Chem & Mol Engn, Inst Fine Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Natl Ctr Drug Screening, 189 Guo Shoujing Rd, Shanghai 201203, Peoples R China;[4]Univ Groningen, Fac Math & Nat Sci, Stratingh Inst Chem, Ctr Syst Chem, Nijenborgh 4, NL-9747 AG Groningen, Netherlands;[5]Univ Groningen, Fac Math & Nat Sci, Zernike Inst Adv Mat, Nijenborgh 4, NL-9747 AG Groningen, Netherlands

年份：2017

卷号：8

外文期刊名：NATURE COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000413118000005)】；

基金：This research is supported by the 973 project (2013CB733700), the National Natural Science Foundation of China (21722801, 21402050, 21420102004 and 21572058), the Program of Introducing Talents of Discipline to Universities (B16017), the Fundamental Research Funds for the Central Universities (222201717003) and the Shanghai RisingStar Program (16QA1401400 to X.-P.H.).

语种：英文

摘要：Development of powerful fluorescence imaging probes and techniques sets the basis for the spatiotemporal tracking of cells at different physiological and pathological stages. While current imaging approaches rely on passive probe-analyte interactions, here we develop photochromic fluorescent glycoprobes capable of remote light-controlled intracellular target recognition. Conjugation between a fluorophore and spiropyran produces the photochromic probe, which is subsequently equipped with a glycoligand "antenna" to actively localize a target cell expressing a selective receptor. We demonstrate that the amphiphilic glycoprobes that form micelles in water can selectively enter the target cell to operate photochromic cycling as controlled by alternate UV/Vis irradiations. We further show that remote light conversion of the photochromic probe from one isomeric state to the other activates its reactivity toward a target intracellular analyte, producing locked fluorescence that is no longer photoisomerizable. We envision that this research may spur the use of photochromism for the development of bioimaging probes.