文献类型：期刊文献

英文题名：Intracellular Thiols and Photo-Illumination Sequentially Activate Doubly Locked Molecular Probes for Long-Term Cell Highlighting and Tracking with Precise Spatial Accuracy

作者：Lin, Qiuning[1];Du, Zengmin[2];Yang, Yunlong[1];Fang, Qian[1];Bao, Chunyan[1];Yang, Yi[2];Zhu, Linyong[1]

机构：[1]E China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Inst Fine Chem, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2014

卷号：20

期号：49

起止页码：16314

外文期刊名：CHEMISTRY-A EUROPEAN JOURNAL

收录：;EI(收录号：20145000315104);WOS:【SCI-EXPANDED(收录号:WOS:000345515700038)】；

基金：This research was supported by NSFC (21173078, 51273064, and 21373084), 973 Project (2013CB733700), Shanghai Sci. Tech. Comm. (14YF1404700), the Postdoctoral Fund in China (2014M550221) and the Fundamental Research Funds for the Central University.

语种：英文

外文关键词：cell photolabeling; coumarins; fluoresceins; photoconvertible fluorescence; thiols

摘要：A novel photoconvertible fluorescent probe, which can be activated by intracellular thiols, has been synthesized. Such a molecular probe comprises three parts: a 7-aminocoumarin phototrigger, a thiol-removable energy acceptor, and a caged fluorescein scaffold with intracellular thiols reactivity as the fluorescent reporter. Extracellularly, the energy acceptor blocks the emission of the coumarin that regulates the photocleavage and photoactivation of the fluorescein. Intracelluarly, the high concentration of thiols releases the energy acceptor, thus activating the S1 state of the phototrigger, which emits coumarin blue fluorescence for pre-visualization and liberates the caged green-fluorescent fluorescein to highlight the specific cell upon illumination. Compared to traditional photoactivated organic dyes, the intracellular thiols activated probe requires double activations: one by intracellular thiols and the other by light activation. The dual activations restrict fluorescence precisely inside live cells and at the particular spatial region of light activation, thus a probe with precise spatial accuracy in live cells.