文献类型：期刊文献

英文题名：Gene Silencing Through CRISPR Interference in Bacteria: Current Advances and Future Prospects

作者：Zhang, Riyu[1];Xu, Wensheng[2];Shao, Shuai[1];Wang, Qiyao[1,3,4]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Beijing Agr Univ, Fac Food Sci & Engn, Lab Agr Prod Detect & Control Spoilage Organis &, Beijing, Peoples R China;[3]Shanghai Collaborat Innovat Ctr Biomfg, Shanghai, Peoples R China;[4]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai, Peoples R China

年份：2021

卷号：12

外文期刊名：FRONTIERS IN MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000639606900001)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (32002436 to SS and 31772891 to QW), Shanghai Sailing Program (20YF1411100), China Postdoctoral Science Foundation (2020M671034), and Beijing Municipal Commission of Education (KM20161002 0016).

语种：英文

外文关键词：CRISPR; Cas; gene silencing; essential genes; Tn-seq; CRISPRi screen

摘要：Functional genetic screening is an important method that has been widely used to explore the biological processes and functional annotation of genetic elements. CRISPR/Cas (Clustered regularly interspaced short palindromic repeat sequences/CRISPR-associated protein) is the newest tool in the geneticist's toolbox, allowing researchers to edit a genome with unprecedented ease, accuracy, and high-throughput. Most recently, CRISPR interference (CRISPRi) has been developed as an emerging technology that exploits the catalytically inactive Cas9 (dCas9) and single-guide RNA (sgRNA) to repress sequence-specific genes. In this review, we summarized the characteristics of the CRISPRi system, such as programmable, highly efficient, and specific. Moreover, we demonstrated its applications in functional genetic screening and highlighted its potential to dissect the underlying mechanism of pathogenesis. The recent development of the CRISPRi system will provide a high-throughput, practical, and efficient tool for the discovery of functionally important genes in bacteria.