文献类型：期刊文献

英文题名：Identification and expression profile of odorant-binding proteins in the parasitic wasp Microplitis pallidipes using PacBio long-read sequencing

作者：Zhang, Hao[1];Wang, Jin-Yan[1];Wan, Nian-Feng[2];Chen, Yi-Juan[1];Ji, Xiang-Yun[1];Jiang, Jie-Xian[1]

机构：[1]Shanghai Acad Agr Sci, Shanghai Engn Res Ctr Low Carbon Agr, Ecoenvironm Protect Inst, Shanghai Key Lab Protected Hort Technol, Shanghai 201403, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China

年份：2022

卷号：29

外文期刊名：PARASITE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000880421700003)】；

基金：This work was supported by the Shanghai Agriculture Applied Technology Development Program (Grant No. T20210204), National Natural Science Foundation of China (Grant No. 32001969, 31601641), Natural Science Foundation of Shanghai (Grant No. 20ZR1449000), Shanghai Rising-Star Program (Grant No. 21QB1404100), and SAAS Program for Excellent Research Team (2022-017).

语种：英文

外文关键词：Biological control; Odorant-binding protein; Parasitic insect; Tissue and temporal expression profile; Transcriptome sequencing

摘要：Microplitis pallidipes Szepligeti (Hymenoptera: Braconidae) is an important parasitic wasp of second and third-instar noctuid larvae such as the insect pests Spodoptera exigua, Spodoptera litura, and Spodoptera frugiperda. As in other insects, M. pallidipes has a chemosensory recognition system that is critical to foraging, mating, oviposition, and other behaviors. Odorant-binding proteins (OBPs) are important to the system, but those of M. pallidipes have not been determined. This study used PacBio long-read sequencing to identify 170,980 M. pallidipes unigenes and predicted 129,381 proteins. Following retrieval of possible OBP sequences, we removed those that were redundant or non-full-length and eventually cloned five OBP sequences: MpOBP2, MpOBP3, MpOBP8, MpOBP10, and MpPBP 429, 429, 459, 420, and 429 bp in size, respectively. Each M. pallidipes OBP had six conserved cysteine residues. Phylogenetic analysis revealed that the five OBPs were located at different branches of the phylogenetic tree. Additionally, tissue expression profiles indicated that MpOBP2 and MpPBP were mainly expressed in the antennae of male wasps, while MpOBP3, MpOBP8, and MpOBP10 were mainly expressed in the antennae of female wasps. MpOBP3 was also highly expressed in the legs of female wasps. Temporal profiles revealed that the expression of each M. pallidipes OBP peaked at different days after emergence to adulthood. In conclusion, we identified five novel odorant-binding proteins of M. pallidipes and demonstrated biologically relevant differences in expression patterns.