文献类型：期刊文献

英文题名：Preparation of near-infrared AIEgen-active fluorescent probes for mapping amyloid-β plaques in brain tissues and living mice

作者：Yan, Chenxu[1];Dai, Jianfeng[1];Yao, Yongkang[1];Fu, Wei[1];Tian, He[1];Zhu, Wei-Hong[1];Guo, Zhiqian[1]

机构：[1]East China Univ Sci & Technol, Inst Fine Chem, Feringa Nobel Prize Scientist Joint Res Ctr, Frontiers Sci Ctr Materiobiol & Dynam Chem, Shanghai, Peoples R China

年份：2023

卷号：18

期号：4

起止页码：1316

外文期刊名：NATURE PROTOCOLS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000920609500001)】；

基金：This work was supported by NSFC/China (22225805, 21878087 and 21908060), National Key Research and Development Program (2021YFA0910000), Innovation Program of Shanghai Municipal Education Commission, Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission, grant 2021 Sci & Tech 03-28), Shanghai Municipal Science and Technology Major Project (Grant 2018SHZDZX03), Shanghai Science and Technology Committee Rising-Star Program (22QC1400400) and Programme of Introducing Talents of Discipline to Universities (B16017).

语种：英文

摘要：Fibrillar aggregates of the amyloid-beta protein (A beta) are the main component of the senile plaques found in brains of patients with Alzheimer's disease (AD). Development of probes allowing the noninvasive and high-fidelity mapping of A beta plaques in vivo is critical for AD early detection, drug screening and biomedical research. QM-FN-SO3 (quinoline-malononitrile-thiophene-(dimethylamino)phenylsulfonate) is a near-infrared aggregation-induced-emission-active fluorescent probe capable of crossing the blood-brain barrier (BBB) and ultrasensitively lighting up A beta plaques in living mice. Herein, we describe detailed procedures for the two-stage synthesis of QM-FN-SO3 and its applications for mapping A beta plaques in brain tissues and living mice. Compared with commercial thioflavin (Th) derivatives ThT and ThS (the gold standard for detection of A beta aggregates) and other reported A beta plaque fluorescent probes, QM-FN-SO3 confers several advantages, such as long emission wavelength, large Stokes shift, ultrahigh sensitivity, good BBB penetrability and miscibility in aqueous biological media. The preparation of QM-FN-SO3 takes similar to 2 d, and the confocal imaging experiments for A beta plaque visualization, including the preparation for mouse brain sections, take similar to 7 d. Notably, acquisition and analyses for in vivo visualization of A beta plaques in mice can be completed within 1 h and require only a basic knowledge of spectroscopy and chemistry.