文献类型：期刊文献

英文题名：Development of nitrilase-mediated process for phenylacetic acid production from phenylacetonitrile

作者：Fan, Haiyang[1];Chen, Lifeng[1];Sun, Huihui[2];Wang, Hualei[1];Liu, Qinghai[1];Ren, Yuhong[1];Wei, Dongzhi[1]

机构：[1]East China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Shandong, Peoples R China

年份：2017

卷号：71

期号：10

起止页码：1985

外文期刊名：CHEMICAL PAPERS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000412975400018)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 21406068/B060804), the Fundamental Research Funds for the Central Universities.

语种：英文

外文关键词：Biotransformation; Fed-batch reaction; Fermentation; Nitrilase; Phenylacetic acid

摘要：This study aimed at developing an efficient biotransformation process for phenylacetic acid production from phenylacetonitrile by using recombinant Escherichia coli M15 harboring a double mutant MG nitrilase (I113M/Y199G) from Burkholderia cenocepacia J2315. A yield of 2310 U/mL nitrilase was obtained by fermentation after the optimization of cultivation conditions, with a specific activity of 64 U/mg dcw. The MG nitrilase showed high substrate tolerance and completely hydrolyzed 100 mM phenylacetonitrile in 30 min under optimal conditions. To alleviate substrate inhibition, periodic or continuous batch-feeding of substrate was used during the biotransformation. Up to 164 g/L substrate was completely hydrolyzed in 9 h with continuous batch-feeding using resting cells, corresponding to 400 U/mL of nitrilase activity, and leading to production of 163.4 g/L phenylacetic acid. The hydrolysis process has potential application for phenylacetic acid production on a large scale.