文献类型：期刊文献

英文题名：Improvement of the performance of H₂O₂ oxidation at low working potential by incorporating TTF-TCNQ into a platinum wire electrode for glucose determination

作者：Li, QS; Ye, BC; Liu, BX; Zhong, JJ

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Res Inst Biochem Engn, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Res Inst Biochem, Shanghai 200237, Peoples R China

年份：1999

卷号：14

期号：3

起止页码：327

外文期刊名：BIOSENSORS & BIOELECTRONICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000079593100011)】；

语种：英文

外文关键词：biosensor; polypyrrole film; TTF-TCNQ; glucose determination

摘要：A micro-biosensor was constructed by incorporating the organic conducting salt tetrathiafulvalene-tetracyanoquinodimethane (TTF-TCNQ) into a platinized platinum (Pt) wire and further covering with the electrochemical polymerical heteropolypyrrole film, in which glucose oxidase (GOx) was entrapped. The enzyme electrode can sensitively determine glucose at a low working potential, mainly based on the oxidation of H2O2. The incorporated TTF-TCNQ can significantly improve the oxidation of H2O2 on the electrode, although a part of the TTF-TCNQ functions as a mediator. Compared with the same electrode prepared without TTF-TCNQ incorporated, the TTF-TCNQ modified electrode had better performance characteristics at a working potential of 200 mV (versus SCE). The response time to 90% of the steady value was shortened from about 40 s to less than 10 s, the lower limit of the linear response was greatly extended from about 1.6 mM to 10 mu M, the linear range was shifted from 1.6-10.0 to 0.01-5 mM and the sensitivity was increased from about 1 to 1.5 mu A/mM. The electrode was quite stable. For continuous operation, the electrode could work for about 5 weeks and only lost 60% of its original sensitivity. Stored at 4 degrees C for intermittent determinations, the electrode kept 80% sensitivity for over 6 months. Due to covering the electrode with a non-conductive heteropolypyrrole film, ascorbate, urate and 4-acetamidophenol caused only negligible current response at an applied potential of 200 mV. (C) 1999 Elsevier Science S.A. All rights reserved.