文献类型：期刊文献

英文题名：Highly efficient strategy for enhancing taxoid production by repeated elicitation with a newly synthesized jasmonate in fed-batch cultivation of Taxus chinensis cells

作者：Qian, Zhi-Gang[1]; Zhao, Zhen-Jiang[2]; Xu, Yufang[2]; Qian, Xuhong[2]; Zhong, Jian-Jiang[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China; [2] Shanghai Key Laboratory of Chemical Biology, Institute of Pesticides and Pharmaceuticals, East China University of Science and Technology, Shanghai 200237, China

年份：2005

卷号：90

期号：4

起止页码：516

外文期刊名：Biotechnology and Bioengineering

收录：EI(收录号：2005249161760)

语种：英文

外文关键词：Bioreactors - Biosynthesis - Cell culture - Cultivation - Metabolism - Volumetric analysis

摘要：A highly efficient bioprocessing strategy was developed for enhancing the production of plant secondary metabolites by repeatedly eliciting a fed-batch culture with a newly synthesized powerful jasmonate analog, 2,3-dihydroxypropyl jasmonate (DHPJA). In suspension cultures of a high taxuyunnanine C (Tc)-producing cell line of Taxus chinensis, 100 μM DHPJA was added on day 7 to fed-batch cultures with feeding of 20 g L-1 sucrose on the same day. The synergistic effect of elicitation and substrate feeding on Tc biosynthesis was observed, which resulted in higher Tc accumulation than that by elicitation or sucrose feeding alone. More interestingly, both specific Tc yield (i.e., Tc content) and volumetric yield was further improved by a second addition of 100 μM DHPJA (on day 12) to the fed-batch cultures. In particular, with repeated elicitation and sucrose feeding the Tc volumetric yield was increased to 827 ± 29 mg L-1, which was 5.4-fold higher than that of the nonelicited batch culture. Furthermore, the above novel strategy was successfully applied from shake flask to a 1-L airlift bioreactor. A high Tc production and productivity of 738 ± 41 mg L-1 and 33.2 ± 1.9 mg L-1 d-1 respectively, was achieved, which is higher than previous reports on Tc production in bioreactors. The results suggest that the aforementioned bioprocessing strategy may potentially be applied to other cell culture systems for efficient production of plant secondary metabolites. ? 2005 Wiley Periodicals, Inc.