文献类型：期刊文献

英文题名：Analysis of cephalosporins by hydrophilic interaction chromatography

作者：Liu, Qiaoxia[1];Xu, Lingyan[1];Ke, Yanxiong[1];Jin, Yu[1];Zhang, Feifang[1];Liang, Xinmiao[1,2]

机构：[1]E China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Sch Pharm, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China

年份：2011

卷号：54

期号：3

起止页码：623

外文期刊名：JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000284814300033)】；

基金：This work was sponsored by National Natural Science Funds for Distinguished Young Scholars (20825518) and Important National Science & Technology Specific Projects in the Eleventh Five-year Plan Period (2009ZX09313-003).

语种：英文

外文关键词：Cephalosporins; Hydrophilic interaction chromatography; The Click beta-CD column; Reversed-phase high performance liquid chromatography

摘要：A simple hydrophilic interaction chromatography (HILIC) method was developed to analyze seven cephalosporins. These seven cephalosporins could be separated well on the Click beta-CD column and Atlantis HILIC Silica column. The effects of buffer concentration and pH on the retention under HILIC mode were studied. Except cefepime hydrochloride (4), the retention of other six cephalosporins increased with increasing buffer concentration, while decreased with increasing pH. Different separation selectivities could be observed on the Click beta-CD column and Atlantis HILIC Silica column, and changing pH also resulted in the changing of separation selectivity. The separations of cephalosporins by HILIC and reversed-phase high performance liquid chromatography (RP-HPLC) were compared, and the two separation modes had good orthogonality. In addition, cefotaxime sodium (1) and its degradation were separated well on the Click beta-CD column, which indicated that the Click beta-CD column by HILIC can be used for studying the stability of cephalosporins. (C) 2010 Published by Elsevier B.V.