文献类型：期刊文献

中文题名：豆油对红霉素生物合成的影响及作用机制分析

英文题名：Effect of Soybean Oil on the Biosynthesis of Erythromycin and Its Mechanism

作者：卞晨光[1];宫衡[1];陈长华[1];付水林[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2004

卷号：30

期号：2

起止页码：139

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;EI(收录号：2004268239441);Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；

基金：教育部留学回国人员启动基金资助项目

语种：中文

中文关键词：豆油;红霉素;丙酰辅酶A合成酶;丙酸激酶;柠檬酸;α-酮戊二酸

外文关键词：soybean oil; erythromycin; propionyl-CoA synthetase; propionate kinase; citrate; (α-ketoglutaric) acid

摘要：在摇瓶培养过程中,培养基中加入豆油可使红霉素的效价从3038U/mL(不加豆油)提高到5221U/mL。但对丙酰辅酶A合成酶及红霉素链霉菌H18丙酸激酶的比活力无显著影响。使用HPLC检测发酵液中α-酮戊二酸(α-KG)的含量,结果发现加豆油后α-KG含量有明显提高。据此推测了豆油经过红霉素链霉菌H18代谢后进入红霉素合成的可能途径是:通过三羧酸循环代谢产生琥珀酰辅酶A,琥珀酰辅酶A异构化产生2-甲基丙二酰辅酶A,2-甲基丙二酰辅酶A作为红霉素的前体最终促进红霉素的合成。
Fermentation titer of erythromycin in shake flask with soybean oil increased from 3 038 U/mL (without soybean oil) to 5 221 U/mL in Streptomyces erythreus H18. But it was found that soybean oil in the shake flask had no influence on the specific activity of propionyl-CoA synthetase and propionate (kinase). Analysis with HPLC showed that, the concentration of α-ketoglutaric acid(α-KG) in shake flask (with) soybean oil increased evidently compared with that without soybean oil. So, the metabolic pathway from soybean oil to erythromycin in Streptomyces erythreus H18 was presumed as follows: soybean oil is metabolized through TCA pathway to generate succinyl-CoA, and then, the 2-methylmalonyl-CoA is formed from succinyl-CoA by isomerization. Finally, 2-methylmalonyl-CoA can be used as the precursor for the biosynthesis of erythromycin.