文献类型：期刊文献

英文题名：Effectiveness of pulsed light treatment for degradation and detoxification of aflatoxin B1 and B2 in rough rice and rice bran

作者：Wang, Bei[1,3];Mahoney, Noreen E.[2];Pan, Zhongli[1,2,3];Khir, Ragab[1,4];Wu, Bengang[3];Ma, Haile[3];Zhao, Liming[5]

机构：[1]Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA;[2]USDA ARS, Western Reg Res Ctr, Albany, CA 94710 USA;[3]Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China;[4]Suez Canal Univ, Fac Agr, Dept Agr Engn, Ismailia 41522, Egypt;[5]E China Univ Sci & Technol, Sch Biotechnol, Dept Food Sci & Technol, Shanghai 200237, Peoples R China

年份：2016

卷号：59

起止页码：461

外文期刊名：FOOD CONTROL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000364890000061)】；

基金：The authors thank Farmers' Rice Cooperative for supplying rice samples and the support by International Science and Technology Cooperation Program of Jiangsu (No. BZ2012052).

语种：英文

外文关键词：Rice; Pulsed light; Degradation; Aflatoxin; Anemia sauna; Fluctuation test

摘要：Aflatoxins primarily accumulate in the hull and bran layers of rough rice making these by-products of rice milling unsuitable for animal feed or human consumption. Contaminated rough rice is also a potential source of aflatoxin exposure to workers handling the grain during post-harvest storage and processing. Currently, no technologies are available to remove or detoxify these toxic and mutagenic fungal metabolites from contaminated rough rice. Pulsed light (PL) is a novel technology with the potential to degrade and detoxify aflatoxins in foods and their processing by-products. Rough rice was inoculated with Aspergillus flavus to produce aflatoxin B-1 (AFB(1)) and B-2 (AFB(2)) contamination, followed by PL treatments of 0.52 J/cm(2)/pulse for various durations. A PL treatment time of 80 s reduced AFB(1) and AFB2 in rough rice by 75.0% and 39.2%, respectively; while a treatment time of 15 s reduced AFB(1) and AFB2 in rice bran by 903% and 86.7%, respectively. Since PL treatments result in the degradation of aflatoxins in situ, the toxicity and mutagenic activity of the residual by-products of AFB(1) and AFB(2) after PL treatment were evaluated. Toxicity was estimated using the brine shrimp (Anemia sauna) lethality assay and mutagenicity measured by the fluctuation test with Salmonella typhimurum tester strains TA98 and TA100. The mutagenic activity of AFB(1) and AFB(2) was completely eliminated by PL treatment, while the toxicity of these two aflatoxins was significantly decreased. The obtained results suggest that PL technology has a promising potential to degrade, detoxify, and inactivate the mutagenic activity of aflatoxins in rough rice and rice bran. Published by Elsevier Ltd.