文献类型：期刊文献

中文题名：Construction of developmentally inspired periosteum-like tissue for bone regeneration

作者：Kai Dai[1,2];Shunshu Deng[1,2];Yuanman Yu[1,2];Fuwei Zhu[1,2];Jing Wang[1,2];Changsheng Liu[2,3,4]

机构：[1]State Key Laboratory of Bioreactor Engineering,East China University of Science and Technology,Shanghai,P.R.China;[2]Engineering Research Center for Biomedical Materials of Ministry of Education,East China University of Science and Technology,Shanghai,P.R.China;[3]Key Laboratory for Ultrafine Materials of Ministry of Education,East China University of Science and Technology,Shanghai,P.R.China;[4]Frontiers Science Center for Materiobiology and Dynamic Chemistry,East China University of Science and Technology,Shanghai,P.R.China

年份：2022

卷号：10

期号：1

起止页码：81

中文期刊名：Bone Research

外文期刊名：骨研究（英文版）

收录：Scopus;CSCD:【CSCD2021_2022】；PubMed;

基金：supported by the National Natural Science Foundation of China for Innovative Research Groups(No.51621002);the National Key R&D Program of China(NO.2018YFE0201500),the National Natural Science Foundation of China(No.31870953);the Foundation of Frontiers Science Center for Materiobiology and Dynamic Chemistry(NO.JKVD1211002);the Weigao Project of Chinese Academy of Sciences[NO.(2020)005].

语种：英文

中文关键词：sized;utilized;finally;

摘要：The periosteum, a highly vascularized thin tissue, has excellent osteogenic and bone regenerative abilities. The generation of periosteum-mimicking tissue has become a novel strategy for bone defect repair and regeneration, especially in critical-sized bone defects caused by trauma and bone tumor resection. Here, we utilized a bone morphogenetic protein-2(BMP-2)-loaded scaffold to create periosteum-like tissue(PT) in vivo, mimicking the mesenchymal condensation during native long bone development. We found that BMP-2-induced endochondral ossification plays an indispensable role in the construction of PTs. Moreover, we confirmed that BMP-2-induced PTs exhibit a similar architecture to the periosteum and harbor abundant functional periosteum-like tissue-derived cells(PTDCs), blood vessels, and osteochondral progenitor cells. Interestingly, we found that the addition of chondroitin sulfate(CS), an essential component of the extracellular matrix(ECM), could further increase the abundance and enhance the function of recruited PTDCs from the PTs and finally increase the regenerative capacity of the PTs in autologous transplantation assays, even in old mice. This novel biomimetic strategy for generating PT through in vivo endochondral ossification deserves further clinical translation.