文献类型：期刊文献

英文题名：Enhancement of phototrophic hydrogen production by Rhodobacter sphaeroides ZX-5 using fed-batch operation based on ORP level

作者：Li, Xu[1]; Dai, Zhen-Zhen[1]; Wang, Yong-Hong[1]; Zhang, Si-Liang[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, 130 Meilong Road, Shanghai 200237, China

年份：2011

卷号：36

期号：20

起止页码：12794

外文期刊名：International Journal of Hydrogen Energy

收录：EI(收录号：20113814357032)

语种：英文

外文关键词：Efficiency - Redox reactions - Batch cell culture - Batch data processing - Photobioreactors - Feedback control - Fermentation - Image enhancement

摘要：In this study, a new outer-cycle flat-panel photobioreactor was designed for an anaerobic, photo-fermentation process by Rhodobacter sphaeroides ZX-5. In order to obtain the high hydrogen yield, photo-hydrogen production by fed-batch culture with on-line oxidation-reduction potential (ORP) feedback control was investigated. Meanwhile, the effects of feeding malic acid concentration and pH adjustment on the growth and hydrogen production of R. sphaeroides ZX-5 were studied. In the entire fed-batch culture, biomass (i.e., OD660) rapidly increased up to 1.79 within 18 h, and then OD660 value stayed constant within a range of 1.85-2.18 until the end of the photo-fermentation. The cumulative hydrogen volumes in each phase of fed-batch process were 2339, 1439, 1328, and 510 ml H2/l-culture, respectively. Throughout the entire repeated fed-batch photo-fermentation, the maximum substrate conversion efficiency of 73.03% was observed in the first fed-batch process, obviously higher than that obtained from batch culture process (59.81%). In addition, compared to the batch culture, a much higher maximum hydrogen production rate (102.33 ml H2/l h) was achieved during fed-batch culture. The results demonstrated that photo-hydrogen production using fed-batch operation based on ORP feedback control is a favorable choice of sustainable and feasible strategy to improve phototrophic hydrogen production efficiency. ? 2011, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.