文献类型：期刊文献

中文题名：毕赤酵母发酵液的脱色和重组水蛭素的分离

英文题名：Decolorization and Isolation of Recombinant Hirudin Expressed in the Methylotrophic Yeast Pichia pastoris

作者：周卫斌[1];周祥山[1];张元兴[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2001

卷号：17

期号：6

起止页码：683

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：CSTPCD;;Scopus;北大核心:【北大核心2000】；CSCD:【CSCD2011_2012】；PubMed;

语种：中文

中文关键词：重组水蛭素;脱色;分离;毕赤酵母;表达

外文关键词：recombinant hirudin, decolorization, isolation

摘要：用毕赤酵母表达重组水蛭素的发酵液中含有大量色素和一些杂蛋白。本文对发酵上清的脱色和重组水蛭素的分离进行了探讨。鉴于水蛭素的稳定性 ,用加热法预处理发酵上清取得了满意的效果。对离子交换、疏水层析、凝胶过滤、羟基磷灰石吸附层析以及它们的优化组合进行了研究 ,结果表明 ,阳离子交换层析、凝胶过滤和阴离子交换层析的组合对发酵上清的脱色和重组水蛭素的分离是有效的。
Schemes for decolorization of broth and isolation of recombinant hirudin from the fermentation supernatants of recombinant yeast %Pichia pastoris% were developed to remove a great deal of non-desired proteins and coloring matters.Based on the stability of hirudin,heating is desirable to pre-treat the fermentation broth.Several different column chromatographies,including anion-exchange,cation-exchange,hydrokyapatite adsorption,hydrophobic interaction,gel filtration and their combination were investigated on the efficiencies of decolorization and isolation.Among these methods,the combination of cation-exchange and anion-exchange was found to be a success in both decolorization and isolation of hirudin.