文献类型：期刊文献

英文题名：Towards acetone-uncoupled biofuels production in solventogenic Clostridium through reducing power conservation

作者：Liu, Dong[1,2];Yang, Zhengjiao[1];Wang, Ping[3];Niu, Huanqing[1,2];Zhuang, Wei[1,2];Chen, Yong[1,2];Wu, Jinglan[1,2];Zhu, Chenjie[1,2];Ying, Hanjie[1,2];Ouyang, Pingkai[1,2]

机构：[1]Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, 30 Puzhu South Rd, Nanjing 211816, Jiangsu, Peoples R China;[2]Jiangsu Natl Synerget Innovat Ctr Advance Mat SIC, 30 Puzhu South Rd, Nanjing 211816, Jiangsu, Peoples R China;[3]East China Univ Sci & Technol, Biomed Nanotechnol Ctr, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2018

卷号：47

起止页码：102

外文期刊名：METABOLIC ENGINEERING

收录：;EI(收录号：20181204922303);WOS:【SCI-EXPANDED(收录号:WOS:000433423600011)】；

基金：The authors thank Prof. Nigel P. Minton from the University of Nottingham for kindly providing the ClosTron plasmids, and Prof. Sheng Yang from Shanghai Institutes for Biological Sciences for kindly providing pSY8 plasmid. This work was supported by the Jiangsu Provincial Natural Science Foundation of China (Grant No.: BK20150938); the National Natural Science Foundation of China (Grant No.: 21706123); the National Basic Research Program of China (973) (2013CB733602); the Major Research Plan of the National Natural Science Foundation of China (21390204); the key program of the National Natural Science Foundation of China (21636003); the Program for Changjiang Scholars and Innovative Research Team in University (IRT_14R28); the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and the Jiangsu Synergetic Innovation Center for Advanced Bio-Manufacture.

语种：英文

外文关键词：Redox cofactor; NADH; Atom economy; Biobutanol; Clostridium acetobutylicum; 2,3-butanediol

摘要：Microbial production of butanol by solventogenic Clostridium has long been complicated with the formation of acetone as an unwanted product, which causes poor product yields and creates a most important problem concerning substrate transformation. Intensive attempts concentrate on carbon conversion pathways to eliminate acetone, but have actually achieved little so far. Here, we believe microbial product distribution can largely depend on how the cell plays its energetic cofactors in central metabolism, and demonstrate that by introducing a synthetic 2,3-butanediol synthesis pathway in Clostridium acetobutylicum as an NADH-compensating module to readjust the reducing power at a systems level, the production of acetone can be selectively and efficiently eliminated (< 0.3 g/L). H-2 evolution was reduced by 78%, and the total alcohol yield was strikingly increased by 19% to 0.44 g/g glucose, much higher than those yet reported for butanol fermentation. These findings highlight that it is the loss of reducing power rather than typically manipulated solventogenesis genes that dominates acetone formation. Further study revealed that the NADH-module triggered apparent regulation of pathways involved in electron transfer and reducing power conservation. The study also suggested the key to conservation of intracellular reducing power might essentially lie in the intermediate processes in central metabolism that are related to redox partners, butyrate or C-4 branches, and possibly NADH and NADPH specificity. This study represents the first effective redox-based configuration of C. acetobutylicum and provides valuable understandings for redox engineering of native Clostridium species towards advanced production of biofuels and alcohols.