文献类型：期刊文献

英文题名：Bright and Stable Cyan Fluorescent RNA Enables Multicolor RNA Imaging in Live Escherichia coli

作者：Jiang, Li[1,2];Zuo, Fangting[1,3,4];Pan, Yuanyuan[1];Li, Ruilong[1];Shi, Yajie[1];Huang, Xinyi[2];Zhang, Dasheng[1];Zhuang, Yingping[4];Zhao, Yuzheng[1,3,5];Lin, Qiuning[2];Yang, Yi[1,3];Zhu, Linyong[1,2];Chen, Xianjun[1,3,5]

机构：[1]East China Univ Sci & Technol, Optogenet & Synthet Biol Interdisciplinary Res Ctr, State Key Lab Bioreactor Engn, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[2]Shanghai Jiao Tong Univ, Sch Biomed Engn, 800 Dong Chuan Rd, Shanghai 200240, Peoples R China;[3]East China Univ Sci & Technol, Shanghai Frontiers Sci Ctr Optogenet Tech Cell Met, Sch Pharm, 130 Mei Long Rd, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Sch Bioengn, Shanghai 200237, Peoples R China;[5]Chinese Acad Med Sci, Res Unit New Tech Live Cell Metab Imaging, Beijing 100730, Peoples R China

年份：2025

卷号：21

期号：9

外文期刊名：SMALL

收录：;EI(收录号：20244417284827);WOS:【SCI-EXPANDED(收录号:WOS:001343829700001)】；

基金：L.J., F.Z., and Y.P. contributed equally to this work. This research was supported by the National Key Research and Development Program of China (2022YFC3400100 to X.C. and Y.Y.; 2019YFA0110500 to L.Z.; 2019YFA0904800 to Y.Y. and L.Z.), NSFC (32250009 to X.C.; 32121005, 32150028, 21937004, and 91857202 to Y.Y.; 21907029 and 21877037 to L.Z.), STI2030-Major Projects (2021ZD0202200 and 2021ZD0202203 to X.C.), the Shanghai Municipal Education Commission (2021 Sci & Tech 03-28), the Shanghai Science and Technology Commission (23J21900400 to X.C.), the State Key Laboratory of Bioreactor Engineering (to X.C. and Y.Y.), the Fundamental Research Funds for the Central Universities (to X.C. and Y.Y.).

语种：英文

外文关键词：bioorthogonality; cyan fluorescent RNA; fluorophore; RNA aptamer; RNA imaging

摘要：Fluorescent RNAs (FRs), which are RNA aptamers that bind and activate their cognate small fluorogenic dyes, have provided a particularly useful approach for imaging RNAs in live cells. Although the color palette of FRs is greatly expanded, a bright and stable cyan FR with good biocompatibility and biorthogonality with currently available FRs remains desirable but is not yet developed. Herein, the development of Myosotis is described, an RNA aptamer that emits bright cyan fluorescence upon binding a novel GFP chromophore-like fluorophore called DBT. Myosotis has a nanomolar affinity for DBT and shows a weak dependence on magnesium for folding. Further studies reveal that the Myosotis-DBT complex has a long fluorescence lifetime, good photostability, and enhance cellular brightness. It is further shown that Myosotis-DBT is biorthogonal to Pepper and Clivia FRs, allowing multiplex fluorescence imaging of RNA in live bacteria. Myosotis can also use to image mRNA in live bacteria, revealing potential coupling between mRNA translation and stability. It is believed that this cyan FR will be a useful tool for studying the functionality and mechanism of RNA underlying diverse biological processes.