文献类型：期刊文献

英文题名：Simultaneous Production of Cellulase and ?-Carotene in the Filamentous Fungus Trichoderma reesei

作者：Li, Jing[1];Chen, Yumeng[1];Gao, Ao[1];Wei, Liujing[1];Wei, Dongzhi[1];Wang, Wei[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2023

卷号：71

期号：16

起止页码：6358

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20231613901935);WOS:【SCI-EXPANDED(收录号:WOS:000970564600001)】；

基金：Funding This research was supported by the National Key Research and Development Program of China (2022YFA0912300) , the Natural Science Foundation of Shanghai (No. 22ZR1417600) , the National Natural Science Foundation of China (32000050) , and the Chenguang Program of the Shanghai Education Development Foundation and Shanghai Municipal Education Commission (21CGA34) .

语种：英文

外文关键词：Trichoderma reesei; carotenoid; ig -carotene; cellulase; waste mycelium

摘要：ig-Carotene is an indispensable additive in beverage, cosmetic, feed, and pharmaceutical production. The fermentation industry annually generates abundant waste mycelia from Trichoderma reesei (T. reesei), a pivotal industrial strain for cellulase and heterologous protein production. In this study, we constructed a T. reesei cell factory for ig-carotene production for the first time. Four key enzymes, CarRP, CarB, GGS1/CrtE, and HMG1, were overexpressed in T. reesei. The concentrations of medium components, including tryptone and glucose, were optimized. The modified strain accumulated ig-carotene at a titer of 218.8 mg/L in flask culture. We achieved cellulase production (FPase, 22.33 IU/mL) with the concomitant production of ig-carotene (286.63 mg/L) from T. reesei in a jar. Overall, this study offers a novel and unique approach to address the costly waste mycelium management process using T. reesei industrial strains that simultaneously produce proteins and carotenoids.