文献类型：期刊文献

英文题名：Miniaturized high throughput detection system for capillary array electrophoresis on chip with integrated light emitting diode array as addressed ring-shaped light source

作者：Ren, Kangning[1];Liang, Qionglin[1];Mu, Xuan[2];Luo, Guoan[1,2];Wang, Yiming[1]

机构：[1]Tsinghua Univ, Dept Chem, Key Lab Bioorgan Phosphorus Chem & Chem Biol, Minist Educ, Beijing 100084, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Shanghai 200237, Peoples R China

年份：2009

卷号：9

期号：5

起止页码：733

外文期刊名：LAB ON A CHIP

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000263466600015)】；

基金：This research was supported by projects of the National Basic Research Program (973 Program) of China (2007CB714505, 2005CB523503) and National Natural Science Foundations of China (Grant 20475031, 20805026). The authors thank Dr Bo Yao and Dr Mei Han at Peking University, Beijing, China for their kind help with the writing of the paper.

语种：英文

摘要：A novel miniaturized, portable fluorescence detection system for capillary array electrophoresis (CAE) on a microfluidic chip was developed, consisting of a scanning light-emitting diode (LED) light source and a single point photoelectric sensor. Without charge coupled detector (CCD), lens, fibers and moving parts, the system was extremely simplified. Pulsed driving of the LED significantly increased the sensitivity, and greatly reduced the power consumption and photobleaching effect. The highly integrated system was robust and easy to use. All the advantages realized the concept of a portable micro-total analysis system (m-TAS), which could work on a single universal serial bus (USB) port. Compared with traditional CAE detecting systems, the current system could scan the radial capillary array with high scanning rate. An 8-channel CAE of fluorescein isothiocyanate (FITC) labeled arginine (Arg) on chip was demonstrated with this system, resulting in a limit of detection (LOD) of 640 amol.