文献类型：期刊文献

中文题名：甘草与厚朴复配物对变异链球菌及生物膜形成的抑制作用

英文题名：Antibacterial Property and Biofilm Formation Inhibition of Complex of Glycyrrhiza uralensis Fisch and Magnolia officinalis Extracts to Streptococcus mutans

作者：蔡昀盈[1];吴梦琪[1];夏玮[1];宋金星[1];张文清[1]

机构：[1]华东理工大学化学与分子工程学院,上海200237

年份：2021

卷号：47

期号：2

起止页码：202

中文期刊名：华东理工大学学报（自然科学版）

外文期刊名：Journal of East China University of Science and Technology

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD_E2021_2022】；

基金：上海市联盟计划2018。

语种：中文

中文关键词：甘草;厚朴;抑菌活性;生物膜清除;变异链球菌

外文关键词：Glycyrrhiza uralensis Fisch;Magnolia officinalis;bacteriostatic effect;biofilm clearing;Streptococcus mutans

摘要：比较了甘草和厚朴的提取物以及两者复配物的抑菌效果,并研究了甘草和厚朴的提取物对变异链球菌乳酸脱氢酶活性的影响和生物膜形成的抑制效果。通过抑菌圈、最小抑菌浓度和部分抑菌浓度指数测试抑菌能力,结果显示甘草和厚朴联合使用时对变异链球菌具有协同作用,且当甘草和厚朴提取物的质量比为1∶2(G-M_(1∶2))时抑菌效果最佳。通过变异链球菌生长曲线探究G-M_(1∶2)对变异链球菌的抑菌时期,研究G-M_(1∶2)对乳酸脱氢酶的活性和生物膜形成的影响,结果显示G-M_(1∶2)能有效抑制乳酸脱氢酶(LDH)的活性和生物膜的形成。
The antibacterial activity of different ethanol extracts from Glycyrrhiza uralensis Fisch(G-EA)and Magnolia officinalis(M-EA)to Streptococcus mutans were tested and compared.The effect on the bacteria activity of lactic dehydrogenase(LDH)and the restrain of biofilm of the two ethanol extract compounds were explored as well.The antibacterial abilities of the two ethanol extracts and their complex were investigated through testing the inhibition zone,minimal inhibit concentration(MIC)and fractional inhibitory concentration(FIC).The results showed that FIC between G-EA and M-EA for Streptococcus mutans was 0.375 and possessed a synergistic effect.After the test of MIC values of different mass ratios of G-EA to M-EA,the bacteriostatic effect was the best for the complex against Streptococcus mutans when the mass ratio of G-EA to M-EA was 1∶2(G-M_(1∶2)),and MIC of the complex reached0.32 g/L.According to the growth curve test of Streptococcus mutans,the main bacteriostatic phase of G-M_(1∶2) was its logarithmic growth phase and stationary phase,and the bacteriostatic effect of G-M_(1∶2) to Streptococcus mutans was superior to the tea polyphenol at the same concentration.The effects of G-M_(1∶2) on lactic dehydrogenase(LDH)activity and biofilm formation in Streptococcus mutans were investigated.It demonstrated that G-M_(1∶2) could effectively inhibit LDH activity and biofilm formation,and the inhibition ability was enhanced with the increase in concentration of GM_(1∶2).The test also indicated the minimum biofilm inhibition concentration 50%(MBIC50)of G-M_(1∶2) was 0.08 g/L.