文献类型：期刊文献

中文题名：转瓶内部结构对无血清悬浮培养昆虫细胞的影响

英文题名：Effect of Inner Designs of Spinner Flasks on Serum-free Cultivation of Insect Cells in Suspension

作者：赵佼[1];戴琥[1];谭文松[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室,上海200237

年份：2000

卷号：16

期号：6

起止页码：755

中文期刊名：生物工程学报

外文期刊名：Chinese Journal of Biotechnology

收录：CSTPCD;;Scopus;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；PubMed;

语种：中文

中文关键词：昆虫细胞;无血清;转瓶;悬浮培养

外文关键词：Insect cells,serum free,spinner flask,suspension culture

摘要：The good growth behavior was achieved for a Trichoplusia ni cell line,called Tn 5B1 4(Tn5)cells,in suspension culture by the use of IC-SFM serum free medium developed by our lab.This medium,combined with the specially designed spinner flask with relatively good culture environment,could support the fragile Tn5 cells to grow well even in low inoculum density.The effect of inner designs of spinner flasks on cellular growth and metabolism was discussed on the basis of this culture system.
The good growth behavior was achieved for a Trichoplusia ni cell line,called Tn 5B1 4(Tn5)cells,in suspension culture by the use of IC-SFM serum free medium developed by our lab.This medium,combined with the specially designed spinner flask with relatively good culture environment,could support the fragile Tn5 cells to grow well even in low inoculum density.The effect of inner designs of spinner flasks on cellular growth and metabolism was discussed on the basis of this culture system.