文献类型：期刊文献

英文题名：PepA binds to and negatively regulates esrB to control virulence in the fish pathogen Edwardsiella piscicida

作者：Yin, Kaiyu[1];Peng, Yue[1];Ahmed, Moamer A. H.[1];Ma, Jiabao[1];Xu, Rongjing[4];Zhang, Yuanxing[1,2,3];Ma, Yue[1,2,3];Wang, Qiyao[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China;[4]Yantai Tianyuan Aquat Co Ltd, Yantai, Shandong, Peoples R China

年份：2020

卷号：232

外文期刊名：MICROBIOLOGICAL RESEARCH

收录：;EI(收录号：20195007812061);WOS:【SCI-EXPANDED(收录号:WOS:000526038300003)】；

基金：This work was supported by grants from the National Key Research and Development Program of China (2018YFD0900500 to QYW), the Ministry of Agriculture of China (CARS-47), and the Science and Technology Commission of Shandong and Shanghai Municipality (2017CXGC0103 and 17391902000).

语种：英文

外文关键词：Edwardsiella piscicida; Pull-down; EsrB; Leucyl aminopeptidase; In vivo colonization

摘要：As an important marine fish pathogen, Edwardsiella piscicida infects a broad range of fish species and causes substantial economic losses. The EsrA-EsrB two-component system is essential for the expression of type III and type VI secretion systems (T3/T6SSs), the key virulence determinants in the bacterium. In this study, a pulldown assay with the esrB promoter as bait was performed to identify the upstream regulators of esrB. As a result, PepA, a leucyl aminopeptidase, was identified as a repressor of EsrB and T3/T6SS expression. PepA bound to the esrB promoter region and negatively regulated the production of T3/T6SS proteins in early stages. Moreover, PepA was found to affect the in vivo colonization of E. piscicida in turbot livers through the regulation of EsrB expression. Collectively, our results enhance the understanding of the virulence regulatory network and in vivo colonization mechanism of E. piscicida.