文献类型：期刊文献

英文题名：Cloning and characterization of bagB and bagC, two co-transcribed genes involved in bagremycin biosynthesis in Streptomyces sp Tu 4128

作者：Zhu, Yunxia[1];Xu, Dakui[1];Liao, Siyi[1];Ye, Jiang[1];Zhang, Huizhan[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2013

卷号：63

期号：1

起止页码：167

外文期刊名：ANNALS OF MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000314692400017)】；

语种：英文

外文关键词：Bagremycin; Aldolase; 3-Dehydroquinate synthase; Streptomyces sp Tu 4128

摘要：Bagremycin A and B, produced by Streptomyces sp. Tu 4128, are two antibiotics active against Gram-positive bacteria and fungi. To elucidate the biosynthetic pathway of bagremycins, two genes named bagB and bagC, were cloned and characterized from a bagremycin-producing strain. The deduced protein products of bagB and bagC show high sequence identity with aldolase and 3-dehydroquinate synthase, respectively. The bagC gene is located immediately downstream of the bagB gene and subsequent reverse transcription and polymerase chain reaction analysis revealed that bagB is co-transcribed with bagC. Inactivation of either bagB or bagC resulted in the complete abolishment of bagremycin production in fermented cultures, which suggests that these two genes are involved in the biosynthesis of bagremycins. The results from this study will allow acquisition of the entire biosynthetic cluster for bagremycins and further our understanding of the biosynthetic pathway and mechanism of action of bagremycin.