文献类型：期刊文献

英文题名：Direct electrochemistry of glucose oxidase on screen-printed electrodes through one-step enzyme immobilization process with silica sol-gel/polyvinyl alcohol hybrid film

作者：Zuo, Shaohua[1,2];Teng, Yuanjie[1,2];Yuan, Huihui[3];Lan, Minbo[1,2]

机构：[1]E China Univ Sci & Technol, Res Ctr Anal & Test, Shanghai 200237, Peoples R China;[2]E China Univ Sci & Technol, Inst Adv Mat, Shanghai 200237, Peoples R China;[3]E China Univ Sci & Technol, Minist Educ, Key Lab Ultrafine Mat, Shanghai 200237, Peoples R China

年份：2008

卷号：133

期号：2

起止页码：555

外文期刊名：SENSORS AND ACTUATORS B-CHEMICAL

收录：;EI(收录号：20083011406566);WOS:【SCI-EXPANDED(收录号:WOS:000259268600033)】；

基金：The support of the Science and Technology Commission of Shanghai Municipality (STCSM; No. 065407061) is gratefully acknowledged.

语种：英文

外文关键词：electrochemical biosensor; direct electron transfer; silica sol-gel; screen-printed electrode; glucose oxidase

摘要：A one-step enzyme immobilization process with silica sol-gel/polyvinyl alcohol was described to achieve direct electrochemistry of glucose oxidase on screen-printed electrodes. The immobilized glucose oxidase displays a couple of stable and well-defined redox peaks with an electron transfer rate constant of 8.38 s(-1) and a formal potential of -460 mV (versus SCE) in phosphate buffer (0.05 M. pH 7.0) at a scan rate of 300 mV s-1. The results suggested that conformation and bioactivity of glucose oxidase could be retained efficiently using the proposed immobilization method and the porous structure of screen-printed electrode Surface was helpful for electron communication of glucose oxidase and the electrode. Furthermore, the modified electrode was used as a glucose biosensor, exhibiting a linear response to glucose concentration ranging from 0 to 4.13 mM and a sensitivity of 3.47 mu A mM(-1) cm(-2) at an applied potential of -0.5 V. The detection limit of the biosensor is 9.8 mu M, based on a signal-to-noise ratio of 3. The present work provided a promising strategy for fabricating a novel and disposable mediatorless glucose biosensor, which could be mass-produced through further development. (C) 2008 Elsevier B.V. All rights reserved.