文献类型：期刊文献

中文题名：十二节杆菌胞外脂肪酶的纯化和性质研究

英文题名：Production,purification and properties of an extracellular lipase from Arthrobacter duodecadis

作者：唐芸[1];宋庆训[1];李晓敦[1];沈亚领[1];魏东芝[1]

机构：[1]华东理工大学生物反应器工程国家重点实验室生物化学研究所,上海200237

年份：2007

卷号：37

期号：4

起止页码：45

中文期刊名：工业微生物

外文期刊名：Industrial Microbiology

收录：北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

语种：中文

中文关键词：十二节杆菌;脂肪酶;疏水层析;纯化

外文关键词：Arthrobacter duodecadis; hydrophobic interaction chromatography; lipase;purification

摘要：十二节杆菌发酵得到的胞外脂肪酶,在5L发酵罐经过34h培养,最高酶活可达75 U/mL。通过硫酸铵梯度沉淀和疏水层析纯化,脂肪酶纯化26倍,总得率24.3%。SDS-PAGE显示脂肪酶分子量为33 kD,脂肪酶在40℃和pH 7.0时酶活力最高,同时在24℃经过48h仍保持一半左右的活力。该脂肪酶的酶活受K+,Mg2+激活,而受Zn2+与Co2+的抑制。
After Arthrobacter duodecadis cultured at 28℃ for 34h, the extracellular lipase with enzyme activity of 75U/mL was obtained. The lipase was purified by ammonium sulfate fraction and hydrophobic interaction chromatography to result in a 26-fold purification with 24.3 % of final yield. The molecular weight of the enzyme was determined to be 33 kD by SDS-PAGE, The enzyme exhibited maximum activity at 40℃ and pH 7. 0 and kept above 50% activity over 48 h at 24℃. The enzyme activity was promoted in the presence of K^＋ , Mg^＋＋ and was inhibited by Zn^＋＋ , Co^＋＋.