文献类型：期刊文献

中文题名：当归水溶性成分HPLC指纹图谱研究

英文题名：HPLC fingerprint of the water-soluble constituents in Angelica Sinensis

作者：张敏[1];胡坪[1];罗国安[2];王义明[3]

机构：[1]华东理工大学化学与分子工程学院,上海200237;[2]华东理工大学药学院中药现代化工程中心,上海200237;[3]清华大学生命科学与医学研究院,北京100084

年份：2007

卷号：29

期号：5

起止页码：628

中文期刊名：中成药

外文期刊名：Chinese Traditional Patent Medicine

收录：CSTPCD;;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家高技术研究发展计划(863计划)(课题编号2003AA2Z2021)

语种：中文

中文关键词：当归;指纹图谱;高效液相色谱

外文关键词：Angelica sinensis ; fingerprint ; HPLC

摘要：目的:建立当归水溶性成分的HPLC指纹图谱方法。方法:采用梯度洗脱法,对当归水溶性成分进行HPLC测定,采用Phenomenex Luna C18(250 mm×4.6 mm ID,5μm)色谱柱,以水(0.1%甲酸)-乙腈作为流动相,检测波长254 nm,洗脱流速0.5 mL/min,记录时间65 min。结果:对指纹图谱方法学的精密度、重复性、稳定性考察中特征峰相对峰面积、相对保留时间的RSD均小于5%。本试验把HPLC指纹图谱结合相似度分析和聚类分析用于当归药材的质量评价,较好地表达了产地和用药部位信息。结论:本试验建立的HPLC指纹图谱方法可作为当归药材水溶性成分的质量控制手段。
AIM ： To establish the HPLC fingerprint method of the water-soluble constituents in Angelica Sinensis. METHODS： A Phenomenex Luna C18 column （250 mm×4.6 mm ID, 5μm） was utilized. Water containing 0.1% formic acid and acetonitrile were used as gradient eluents. The detection wavelength was set at 254 nm and the flow rate was 0.5 mL/min. UV spectra was recorded within 65 min. RESULTS ： The RSD of relative peak areas and retention time of peaks in precision, repeatability and stability has not reached 5.0%, respectively. HPLC fingerprint, combined with similarity analysis and cluster method, are used to evaluate the quality of Angelica Sinensis, and it describes the character of regions and fractions properly. CONCLUSION ： The HPLC fingerprint method is practicable as a standard method in controlling the quality of the water-soluble constituents in Angelica Sinensis.