文献类型：期刊文献

英文题名：pH-Activatable tumor-targeting gold nanoprobe for near-infrared fluorescence/CT dual-modal imaging in vivo

作者：Tang, Yao[1];Shi, Hongyuan[2];Cheng, Di[1];Zhang, Jing[2];Lin, Yuncheng[1];Xu, Yufang[1];Qian, Xuhong[1];Zhu, Weiping[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China;[2]Nanjing Med Univ, Affiliated Hosp 1, Dept Radiol, Nanjing 210029, Jiangsu, Peoples R China

年份：2019

卷号：179

起止页码：56

外文期刊名：COLLOIDS AND SURFACES B-BIOINTERFACES

收录：;EI(收录号：20191406722780);WOS:【SCI-EXPANDED(收录号:WOS:000471736600007)】；

基金：This research was supported by grants from the National Natural Science Foundation of China (Grants 21476077, 81701760, 21878088 and 21236002), Science and Technology Project of Jiangsu Province of China (BK20171086), and Key Project of the Shanghai Science and Technology Committee (18DZ1112703).

语种：英文

外文关键词：Nanoprobe; Gold nanoparticles; Tumor microenvironment; Fluorescence imaging; Computed tomography

摘要：One hallmark of solid tumors, regardless of its type or stage, is the existence of an unsual acidic micro environment, which has been considered a specific and ideal target for cancer imaging. Therefore, we developed a pH-activatable nanoprobe GNPs-CKL-FA for near-infrared fluorescence (NIR) and computed tomography (CT) imaging of tumors. This nanoprobe consists of a near-infrared fluorophore (Cy5.5), a pH-sensitive ketal linker, and gold nanoparticles (GNPs) decorated with folates that could bind to tumor cells' surface receptors to promote cellular internalization. This ability of folate to mediate tumor targeting and accelerate internalization has been confirmed by in vitro experiments with HeLa cells. The fluorescence of the nanoprobes successfully activated by low intracellular pH, especially in more acidic organelles. Furthermore, fluorescence signals increased to a greater extent when the pH in tumors was lowered by injection of acetate buffer and isoproterenol. The CT contrast of GNPs-CKL-FA was obtained after administering intravenously to HeLa subcutaneous tumor-bearing mice. These results suggest that GNPs-CKL-FA has the potential to be a pH-activatable fluorescent nanoprobe combined with CT contrast agent for tumor targeted imaging.