文献类型：期刊文献

英文题名：Tartaric acid induced conversion of protopanaxadiol to ginsenosides Rg3 and Rg5 and their in situ recoveries by integrated expanded bed adsorption chromatography

作者：Huang, Dan[1];Li, Yang[2];Zhang, Min[1];Ruan, Shengli[2];Zhang, Hongyang[2];Wang, Yuerong[2];Hu, Ping[2]

机构：[1]East China Univ Sci & Technol, Shanghai Key Lab New Drug Design Modern Engn Ctr, Sch Pharm, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Shanghai Key Lab Funct Mat Chem, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China

年份：2016

卷号：39

期号：15

起止页码：2995

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20163202699198);WOS:【SCI-EXPANDED(收录号:WOS:000383360200013)】；

基金：This research was financially supported by the National Natural Science Foundation of China (Nos. 81273481 and 81573397), the Fundamental Research Funds from the Central Universities, and the open project of State Key Laboratory of Quality Research in Chinese Medicine (Macau University of Science and Technology, MUST-SKL-2016-06) funded by the Macao Science and Technology Development Fund, Macau Special Administrative Region.

语种：英文

外文关键词：Expanded bed adsorption chromatography; Ginsenoside Rg(3); Ginsenoside Rg(5); Tartaric acid; Traditional Chinese medicine

摘要：Panax ginseng has been applied in traditional Chinese medicine for over 2000 years. It is still one of the most popular herbs in recent decades. The prescribed ginseng-containing medicines consist of protopanaxadiol and protopanaxatriol ginsenosides, which are the major constituents of the herb. Minor ginsenosides at low levels in the herb, such as Rg(3) and Rg(5), have attracted more rising attention than the major ones. The existing approaches to prepare Rg(3) and Rg(5) usually rely on either steamed red ginseng as the source or chemical/ enzymatic conversion of protopanaxadiol to the targets. It is still highly desirable to effectively achieve such minor components. In this paper, a method integrated extraction of protopanaxadiol and conversion of it to Rg(3) and Rg(5) has been proposed. Protopanaxadiol was extracted and simultaneously converted to Rg(3) and Rg(5) by D, L-tartaric acid. The targets were absorbed by resins on expanded bed adsorption chromatography and were then separated from other ginsenosides in different stages. Compared with conventional methods, the developed process has advantages in shortening time consumption and improving the conversion ratio of protopanaxadiol, which is promising in directly achieving Rg(3) and Rg(5) from P. ginseng.