文献类型：期刊文献

英文题名：Arylamino-substituted Rhodamine as a Fluorogenic Molecular Rotor for the Wash-free Imaging of Non-catalytic Proteins in Live Cells

作者：Tang, Huiling[1];Yuan, Xia[1];Chen, Yefeng[1];Li, Yuyao[1,2];Xu, Xiaoyong[2];Xie, Hexin[1]

机构：[1]East China Univ Sci & Technol, Sch Pharm, State Key Lab Bioreactor Engn, Shanghai Frontier Sci Res Base Optogenet Tech Cell, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai 200237, Peoples R China

年份：2024

卷号：4

期号：1

外文期刊名：ANALYSIS & SENSING

收录：EI(收录号：20240315395496);WOS:【ESCI(收录号:WOS:001038309300001)】；

基金：Financial supports from the NSFC (22277027 and 22207035), the Fundamental Research Funds for the Central Universities, and Shanghai Frontier Science Research Base of Optogenetic Techniques for Cell Metabolism (Shanghai Municipal Education Commission, grant 2021 Sci & Tech 03-28) are greatly appreciated.

语种：英文

外文关键词：live cell imaging; molecular rotor; protein; integrin alpha(v)beta(3)

摘要：Fluorescent probes are valuable tools to visualize non-catalytic proteins in live cells. Currently, the majority of imaging reagents for non-catalytic proteins are based on "always-on" fluorophores and the use of these reagents usually necessitate a wash step to remove unbounded fluorophores before microscope imaging. Herein, we report the use of arylamino-substituted rhodamine as an activatable fluorophore for the imaging of non-catalytic protein in live cells. We have shown the induction of an arylamino to structurally rigid rhodamine could significantly reduce the fluorescent emission in aqueous medium but the ligand-directed binding of this molecule to protein receptor could effective restrict its intramolecular motion and thus lead to enhancement in fluorescence intensity at 590 nm over 30fold. With fluorescent probes based on this fluorophore, we could visualize integrin av ss 3 and azido-functionalized glycans in living cells with high contrast in a wash-free manner.