文献类型：期刊文献

英文题名：Efficient anaerobic production of succinate from glycerol in engineered Escherichia coli by using dual carbon sources and limiting oxygen supply in preceding aerobic culture

作者：Li, Qing[1];Huang, Bing[1];Wu, Hui[1];Li, Zhimin[1,2];Ye, Qin[1]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2017

卷号：231

起止页码：75

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20170703340840);WOS:【SCI-EXPANDED(收录号:WOS:000400866600010)】；

基金：This work was supported by the National High Technology Research and Development Program of China (No. 2011AA02A203), the National Science Foundation for Young Scientist of China (Grant No. 21406065), the Fundamental Research Funds for the Central Universities (Grant Nos. 222201313007 & 22A201514042). Partially supported by Open Funding Project of the State Key Laboratory of Bioreactor Engineering. Partially supported by the Foundation of Key Laboratory for Industrial Biocatalysis (Tsinghua University), Ministry of Education (Grant No. 2015101). We thank Dr. Yipeng Wang (Rice University) for reading through the manuscript.

语种：英文

外文关键词：Succinate; Glycerol; Acetate; Escherichia coli; Two-stage fermentation

摘要：Glycerol is an important resource for production of value-added bioproducts due to its large availability from the biodiesel industry as a by-product. In this study, two metabolic regulation strategies were applied in the aerobic stage of a two-stage fermentation to achieve high metabolic capacities of the pflB ldhA double mutant Escherichia coli strain overexpressing phosphoenolpyruvate carboxykinase (PCK) in the subsequent anaerobic stage: use of acetate as a co-carbon source of glycerol and restriction of oxygen supply in the PCK induction period. The succinate concentration achieved 926.7 mM with a yield of 0.91 mol/mol during the anaerobic stage of fermentation in a 1.5-L reactor. qRT-PCR indicated that the two strategies enhanced transcription of genes related with glycerol metabolism and succinate production. Our results showed this metabolically engineered E.coli strain has a great potential in producing succinate using glycerol as carbon source. (C) 2017 Elsevier Ltd. All rights reserved.