文献类型：期刊文献

英文题名：Stimulation of Bathyarchaeota in enrichment cultures by syringaldehyde 4-hydroxybenzaldehyde and vanillin under anaerobic conditions

作者：Lin, Dan-Dan[1,2,4];Liu, Yi-Fan[1,2,3,4];Zhou, Lei[1,2,4];Yang, Shi-Zhong[1,2,3];Gu, Ji-Dong[5];Mu, Bo-Zhong[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr MEOR, 130 Meilong Rd, Shanghai 200237, Peoples R China;[4]Shanghai Inst Pollut Control & Ecol Secur, Shanghai 200092, Peoples R China;[5]Guangdong Technion Israel Inst Technol, Environm Sci & Engn Res Grp, 241 Daxue Rd, Shantou 515063, Guangdong, Peoples R China

年份：2022

卷号：171

外文期刊名：INTERNATIONAL BIODETERIORATION & BIODEGRADATION

收录：;EI(收录号：20221511953577);WOS:【SCI-EXPANDED(收录号:WOS:000793195600003)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant No. 42173076, 52074129 and 42061134011) , the Natural Science Foundation of Shanghai (Grant No. 21ZR1417400) , Fundamental Research Funds for the Central Universities (No. JKJ01221716) and the Research Program of State Key Laboratory of Bioreactor Engineering to Y.F.L. and B.Z.M, the NSFC/RGC Joint Research Fund (No. 41161160560) to J.D.G.

语种：英文

外文关键词：Bathyarchaeota; Soil sediment; Aldehyde compound; Biodegradation

摘要：Bathyarchaeota covering about 25 subgroups are a newly discovered archaeal phylum with widespread in anaerobic environments including marine sediments, soils and subsurface petroleum reservoirs. Although recent studies showed that Bathyarchaeota can use lignin as an energy source to get enrichment, there is a lack of the pure culture and thus the understanding about Bathyarchaeota is still based on genomic information. In this study, lignin and three aldehyde compounds, namely syringaldehyde, 4-hydroxybenzaldehyde, and vanillin, which were recognized as the metabolic intermediates of lignin biodegradation, were used as substrates to enrich Bathyarchaeota from mangrove sediments, and enrichment cultures of Bathyarchaeota was successfully established in the laboratory. The changes in gene-copy numbers, microbial composition and consumption of the substrates indicated that the syringaldehyde significantly stimulated the growth of Bathyarchaeota in the enrichment culture. Meanwhile, the relative abundance of Bathyarchaeota was as high as 97.13% in term of the archaeal composition, especially bathyarchaeotal genome subgroup-6 accounted for more than 99.9% in Bathyarchaeota, and 4.74 mmol L-1 methane were also detected in the enrichment culture amended with syringaldehyde as a substrate after about 117 days of incubation, compared to 4-HBA (3.75 mmol L-1) and VA (3.75 mmol L-1). This work will facilitate the design of high-efficiency enrichment cultures and understanding about the metabolic and ecological functions of Bathyarchaeota in nature.