文献类型：期刊文献

英文题名：Purification of compounds from Lignum Dalbergia Odorifera using two-dimensional preparative chromatography with Click oligo (ethylene glycol) and C18 column

作者：Feng, Jiatao[1];Xiao, Yuansheng[1];Guo, Zhimou[1];Yu, Danhua[2];Jin, Yu[2];Liang, Xinmiao[1,2]

机构：[1]Chinese Acad Sci, Dalian Inst Chem Phys, Grad Sch, Dalian 116023, Peoples R China;[2]E China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China

年份：2011

卷号：34

期号：3

起止页码：299

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000287426000009)】；

基金：This work was sponsored by National Natural Science Funds for Distinguished Young Scholars (20825518), Important National Science & Technology Specific Projects in the Eleventh Five-year Plan Period (2009ZX09313-003) and 111 Project (B07023).

语种：英文

外文关键词：Click oligo (ethylene glycol) column; C18 column; Lignum Dalbergia Odorifera; Traditional Chinese medicine; Two-dimensional preparation

摘要：Purification of compounds from traditional Chinese medicines (TCMs) is an important task for understanding the chemical composition of TCMs. However, it is difficult to obtain compounds with high enough purity for identification by NMR due to the complexity of TCMs in chemical composition. In this study, a two-dimensional purification method based on a Click oligo (ethylene glycol) column and a C18 column was developed to realize an orthogonal separation in preparative level for purifying compounds efficiently. The first dimensional preparation was performed on a Click oligo (ethylene glycol) column to simplify the sample into the fractions with good separation repeatability. On the first dimension, 7.2 g sample was separated into 11 fractions with a recovery of 86% within 6 h. A C18 column was taken as the second dimension to realize the high-performance separation and rapid preparation from the fractions collected from the first dimension. Eight compounds in fraction 6 and 2 compounds in fraction 8 were isolated and identified after optimizing the separation and collection parameters. This method is a high-efficient and orthogonal preparation method to improve the separation of a complex sample and increase the purity of the compounds, which benefits from the application of novel materials in the preparation and purification.