文献类型：期刊文献

英文题名：Single-Cell Transcriptomic Analysis Reveals Neutrophil as Orchestrator during b-Glucan-Induced Trained Immunity in a Teleost Fish

作者：Mu, Di[1];Yang, Jin[1];Jiang, Yu[1];Wang, Zhuang[1];Chen, Weijie[1];Huang, Jianchang[1];Zhang, Yuanxing[2];Liu, Qin[1,3,4];Yang, Dahai[1,4,5]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai, Peoples R China;[2]Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai, Peoples R China;[3]Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China;[4]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai, Peoples R China;[5]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China

年份：2022

卷号：209

期号：4

起止页码：783

外文期刊名：JOURNAL OF IMMUNOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000886988000016)】；

基金：This work was supported by the National Natural Science Foundation of China (32122090 to D.Y.) , the Natural Science Foundation of Shanghai (20ZR1415500 to D.Y.) , and the Fundamental Research Funds for the Central Universities (222201211626) .

语种：英文

摘要：Trained immunity defines long-term memory of innate immunity based on transcriptional, epigenetic, and metabolic modifications of myeloid cells, which are characterized by elevated proinflammatory responses toward homologous or heterologous secondary stimuli in mammals. However, the evidence of trained immunity-associated immune cells and its molecular mechanism in teleost fish remains largely unknown. In this study, we established a trained immunity activation model in turbot (Scophthalmus maximus) and found that administration with beta-glucan induces protection against a bacterial infection. Through single-cell RNA sequencing to annotate 14 clusters of innate and adaptive immune cells, as well as two clusters of blood cells, from head kidney and spleen, respectively, we characterized that neutrophil displays cardinal features of trained immunity by analyzing the expression abundance of trained immunity database -related genes at the single-cell level. Subsequently, through establishing an in vivo training and in vitro neutrophil challenge model, we found that the trained neutrophils exhibit a significant elevation of the IL-1R signaling pathway after Edwardsiella piscicida infection. Furthermore, inhibition of neutrophil's IL-1R signaling pathway through anakinra treatment impaired the heightened production of reactive oxygen, nitrogen species, lactate, as well as the neutrophil extracellular traps formation and bacterial killing ability. Taken together, these findings characterized neutrophil as the orchestrator to express features of trained immunity, and revealed that the IL-1R signaling pathway plays a critical role in induction of trained immunity for bacterial clearance in teleost fish.