文献类型：期刊文献

英文题名：Hydrophilic phytic acid-functionalized magnetic dendritic mesoporous silica nanospheres with immobilized Ti4+: A dual-purpose affinity material for highly efficient enrichment of glycopeptides/phosphopeptides

作者：Hong, Yayun[1];Zhan, Qiliang[1];Zheng, Yu[1];Pu, Chenlu[1];Zhao, Hongli[1];Lan, Minbo[1,2]

机构：[1]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai Key Lab Funct Mat Chem, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

年份：2019

卷号：197

起止页码：77

外文期刊名：TALANTA

收录：;EI(收录号：20190206360896);WOS:【SCI-EXPANDED(收录号:WOS:000460710200010)】；

基金：This research was financially supported by the Science and Technology Commission of Shanghai Municipality (STCSM, No. 16520710800) and the Fundamental Research Funds for the Central Universities (No. 222201817022).

语种：英文

外文关键词：Magnetic mesoporous silica microspheres; Phytic acid; LbL assembly; N-glycopeptides enrichment; Phosphopeptide enrichment

摘要：In this work, magnetic mesoporous silica microspheres (Mag-MSMs) with ordered radial mesochannels were fabricated by a self-assembly synthesis in chlorobenzene-water mixed system. Then, the obtained Mag-MSMs were modified with polyethyleneimine (PEI), phytic acid (PA) and Ti4+ (denoted as Mag-MSMs@PEI-PA-Ti4+) via layer by layer (LbL) assembly. Due to the excellent hydrophilicity of PEI and PA and the large amount of Ti4+, the Mag-MSMs@PEI-PA-Ti4+ possessed combined properties of hydrophilic interaction liquid chromatography (HILIC)- and immobilized metal ion affinity chromatography (IMAC)-based materials, which could be used as a dual-purpose material for N-glycopeptides or phosphopeptides enrichment. The proposed Mag-MSMs@PEI-PA-Ti4+ exhibited an outstanding performance for N-glycopeptides enrichment (selectivity: IgG/BSA = 1:1000; sensitivity: 0.5 fmol/mu L. IgG) and phosphopeptides enrichment (selectivity: alpha-casein/BSA = 1:5000; sensitivity: 0.2 fmol/mu L alpha-casein). Furthermore, after enrichment with Mag-MSMs@PEI-PA-Ti4+, a total of 276 N-glycopeptides assigned to 132 glycoproteins were identified from 2 mu L human serum and 1645 phosphopeptides corresponding to 704 phosphoproteins were identified from 200 mu g HeLa cell extracts.