文献类型：期刊文献

英文题名：Hydrophilic interaction liquid chromatography for the separation, purification, and quantification of raffinose family oligosaccharides from Lycopus lucidus Turcz

作者：Liang, Tu[1];Fu, Qing[1];Li, Fangbing[1];Zhou, Wei[1];Xin, Huaxia[1];Wang, Hui[1];Jin, Yu[1];Liang, Xinmiao[1,2]

机构：[1]E China Univ Sci & Technol, Sch Pharm, Engn Res Ctr Pharmaceut Proc Chem, Minist Educ, Shanghai 200237, Peoples R China;[2]Chinese Acad Sci, Dalian Inst Chem Phys, Key Lab Separat Sci Analyt Chem, Key Lab Nat Med, Dalian 116023, Peoples R China

年份：2015

卷号：38

期号：15

起止页码：2607

外文期刊名：JOURNAL OF SEPARATION SCIENCE

收录：;EI(收录号：20152701000102);WOS:【SCI-EXPANDED(收录号:WOS:000358443600006)】；

基金：This work was supported by the Specialized Research Fund for the Doctoral Program of Higher Education of China (grant no. 20130074120017), the Natural Science Foundation of Shanghai, China (grant no. 13ZR1453200), and National Natural Science Funds (21005028).

语种：英文

外文关键词：Hydrophilic interaction liquid chromatography; Lycopus lucidus Turcz.; Oligosaccharides; Purification; Quantitation

摘要：A systematic strategy based on hydrophilic interaction liquid chromatography was developed for the separation, purification and quantification of raffinose family oligosaccharides from Lycopus lucidus Turcz. Methods with enough hydrophilicity and selectivity were utilized to resolve the problems encountered in the separation of oligosaccharides such as low retention, low resolution and poor solubility. The raffinose family oligosaccharides in L. lucidus Turcz. were isolated using solid-phase extraction followed by hydrophilic interaction liquid chromatography at semi-preparative scale to obtain standards of stachyose, verbascose and ajugose. Utilizing the obtained oligosaccharides as standards, a quantitative determination method was developed, validated and applied for the content determination of raffinose family oligosaccharides both in the aerial and root parts of L. lucidus Turcz. There were no oligosaccharides in the aerial parts, while in the root parts, the total content was 686.5 mg/g with the average distribution: raffinose 66.5 mg/g, stachyose 289.0 mg/g, verbascose 212.4 mg/g, and ajugose 118.6 mg/g. The result provided the potential of roots of L. lucidus Turcz. as new raffinose family oligosaccharides sources for functional food. Moreover, since the present systematic strategy is efficient, sensitive and robust, separation, purification and quantification of oligosaccharides by hydrophilic interaction liquid chromatography seems to be possible.