文献类型：期刊文献

英文题名：Optimizing the medium compositions for accumulation of the novel FR-008/Candicidin derivatives CS101 by a mutant of Streptomyces sp. using statistical experimental methods

作者：Mao, Xiangzhao[1]; Shen, Yaling[1]; Yang, Liang[1]; Chen, Shi[2]; Yang, Yuping[3]; Yang, Jingya[4]; Zhu, Hu[1]; Deng, Zixin[2]; Wei, Dongzhi[1]

机构：[1] State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai, 200237, China; [2] Laboratory of Microbial Metabolism, School of Life Science and Biotechnology, Shanghai Jiaotong University, Shanghai, 200030, China; [3] Department of Mathematics, East China University of Science and Technology, Shanghai, 200237, China; [4] College of Food Science, Shanghai Fisheries University, Shanghai, 200090, China

年份：2007

卷号：42

期号：5

起止页码：878

外文期刊名：Process Biochemistry

收录：EI(收录号：20071510538357)

语种：英文

外文关键词：Antibiotics - Concentration (process) - Derivatives - Mathematical models - Optimization - Statistical methods

摘要：Medium compositions for the production of the novel derivatives of FR-008/Candicidin which was produced by a mutant of Streptomyces sp. FR-008 were optimized using two statistical methods including Plackett-Burman design (P-B), which was applied to find the key ingredients for the best medium composition, and response surface methodology (RSM), which was used to determine the optimal concentrations of these components. Results indicated that peptone, copper sulfate and glycerol had significant effects on the production. Under the proposed optimized conditions, the CS101 experimental yield (191.259 mg/L) closely matched the yield (203.536 mg/L) predicted by the statistical model. The optimization of the medium contributed to 10-fold higher antibiotic production than that of the control. It was first revealed that copper could stimulate FR-008/Candicidin and their derivatives synthesis at an optimal concentration in this paper, moreover, the basis of this phenomenon was also explained by investigating the enhancement of the enzymatic pathways. ? 2007 Elsevier Ltd. All rights reserved.