文献类型：期刊文献

英文题名：A versatile biosensing platform coupling CRISPR-Cas12a and aptamers for detection of diverse analytes

作者：Zhao, Xiangxiang[1,2];Li, Shanshan[3];Liu, Guang[1,2];Wang, Zhong[4,5];Yang, Zhiheng[1,2];Zhang, Quanwei[1,2,4];Liang, Mindong[1,2];Liu, Jiakun[6];Li, Zilong[4];Tong, Yaojun[7];Zhu, Guoliang[1,2];Wang, Xinye[1,2];Jiang, Lan[1,2];Wang, Weishan[1,2,4,8];Tan, Gao-Yi[1,2];Zhang, Lixin[1,2]

机构：[1]East China Univ Sci & Technol ECUST, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol ECUST, Sch Biotechnol, Shanghai 200237, Peoples R China;[3]Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China;[4]Chinese Acad Sci, Inst Microbiol, State Key Lab Microbial Resources, Beijing 100101, Peoples R China;[5]Shanghai Jiao Tong Univ, Dept Urol, Sixth Peoples Hosp South Campus, Shanghai 201499, Peoples R China;[6]Chinese Acad Sci, Shenzhen Inst Synthet Biol, Shenzhen Inst Adv Technol, Shenzhen 518055, Peoples R China;[7]Tech Univ Denmark, Novo Nordisk Fdn Ctr Biosustainabil, DK-2800 Lyngby, Denmark;[8]Univ Chinese Acad Sci, Beijing 100049, Peoples R China

年份：2021

卷号：66

期号：1

起止页码：69

外文期刊名：SCIENCE BULLETIN

收录：;EI(收录号：20204409415801);WOS:【SCI-EXPANDED(收录号:WOS:000608377500013)】；

基金：This work was supported by the National Natural Science Foundation of China (31770055, 31922002, 31720103901, and 31772242), the 111 Project (B18022), the Fundamental Research Funds for the Central Universities (22221818014), the Shanghai Science and Technology Commission (18JC1411900), the Young Scientists Innovation Promotion Association of Chinese Academy of Sciences (2016087) to Weishan Wang, the Shandong Taishan Scholar Program of China to Lixin Zhang, and the Open Project Funding of the State Key Laboratory of Bioreactor Engineering.

语种：英文

外文关键词：CRISPR-Cas12a; Aptamer; Biosensing platform; Diverse analyte; Alpha fetoprotein detection; Cocaine detection

摘要：Rapid and sensitive detection of various analytes is in high demand. Apart from its application in genome editing, CRISPR-Cas also shows promises in nucleic acid detection applications. To further exploit the potential of CRISPR-Cas for detection of diverse analytes, we present a versatile biosensing platform that couples the excellent affinity of aptamers for broad-range analytes with the collateral single-strand DNA cleavage activity of CRISPR-Cas12a. We demonstrated that the biosensors developed by this platform can be used to detect protein and small molecule in human serum with a complicated background, i.e., the tumor marker alpha fetoprotein and cocaine with the detection limits of 0.07 fmol/L and 0.34 mu mol/L, respectively, highlighting the advantages of simplicity, sensitivity, short detection time, and low cost compared with the state-of-the-art biosensing approaches. Altogether, this biosensing platform with plug-and-play design show great potential in the detection of diverse analytes. (C) 2020 Science China Press. Published by Elsevier B.V. and Science China Press. All rights reserved.