文献类型：期刊文献

英文题名：Rectifying artificial nanochannels with multiple interconvertible permeability states

作者：Qian, Ruocan[1,2,3,4];Wu, Mansha[1,2,3,4];Yang, Zhenglin[5];Wu, Yuting[5];Guo, Weijie[6];Zhou, Zerui[1,2,3,4];Wang, Xiaoyuan[1,2,3,4];Li, Dawei[1,2,3,4];Lu, Yi[5,6]

机构：[1]East China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Joint Int Lab Precis Chem, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Frontiers Sci Ctr Materiobiol & Dynam Chem, Shanghai 200237, Peoples R China;[4]East China Univ Sci & Technol, Sch Chem & Mol Engn, Shanghai 200237, Peoples R China;[5]Univ Texas Austin, Dept Chem, Austin, TX 78712 USA;[6]Univ Texas Austin, Dept Mol Biosci, Austin, TX 78712 USA

年份：2024

卷号：15

期号：1

外文期刊名：NATURE COMMUNICATIONS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001180826600014)】；

基金：This research was supported by the National Natural Science Foundation of China (21977031), Science and Technology Commission of Shanghai Municipality (2018SHZDZX03), Shanghai Science and Technology Committee (22ZR1416800, 23ZR1416100), and the Fundamental Research Funds for the Central Universities to R.Q., and the U.S. National Institutes of Health (GM141931) to Y.L. Thanks eceshi (www.eceshi.com) for the Zeta analysis.

语种：英文

摘要：Transmembrane channels play a vital role in regulating the permeation process, and have inspired recent development of biomimetic channels. Herein, we report a class of artificial biomimetic nanochannels based on DNAzyme-functionalized glass nanopipettes to realize delicate control of channel permeability, whereby the surface wettability and charge can be tuned by metal ions and DNAzyme-substrates, allowing reversible conversion between different permeability states. We demonstrate that the nanochannels can be reversibly switched between four different permeability states showing distinct permeability to various functional molecules. By embedding the artificial nanochannels into the plasma membrane of single living cells, we achieve selective transport of dye molecules across the cell membrane. Finally, we report on the advanced functions including gene silencing of miR-21 in single cancer cells and selective transport of Ca2+ into single PC-12 cells. In this work, we provide a versatile tool for the design of rectifying artificial nanochannels with on-demand functions. Transmembrane channels have inspired the development of biomimetic channels. Here, the authors present a class of artificial nanochannels based on DNAzyme-functionalized glass nanopipettes to allow for the control of channel permeability.