文献类型：期刊文献

英文题名：Pilot-scale biodesulfurization of natural gas: microbial ecology and community dynamics of an in situ enriched system

作者：Wang, Biao[1,2,3,4,5];Yang, Fan[4,6];Zhou, Lei[1,2];Li, Zhuang-zhuang[4,6];Zhang, Songdi[7];Zhang, Na[7];Liang, Jinsong[7];Mu, Bo-Zhong[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[2]East China Univ Sci & Technol, Sch Chem & Mol Engn, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]East China Univ Sci & Technol, Engn Res Ctr Microbial Enhanced Oil Recovery, Shanghai 200237, Peoples R China;[4]Jiangsu Prov Engn Res Ctr Oil & Gas Microbial Tech, Yangzhou 225009, Peoples R China;[5]Yangzhou Petrochem Co Ltd, Yangzhou 225267, Peoples R China;[6]Sinopec, Res Inst Petr Engn Technol, Jiangsu Oilfield, Yangzhou 225009, Peoples R China;[7]Harbin Inst Technol, Sch Ecoenvironm, Shenzhen 518055, Peoples R China

年份：2026

卷号：137

期号：6

外文期刊名：JOURNAL OF APPLIED MICROBIOLOGY

收录：;EI(收录号：20262620973653);WOS:【SCI-EXPANDED(收录号:WOS:001799810900001)】；

基金：This work was supported by Sinopec Scientific Research Project: Research and Application of Biological Desulfurization Technology for Oilfield Associated Gas (Project No. P22028).

语种：英文

外文关键词：natural gas; biodesulfurization; sulfur recovery; indigenous microbes; sulfur-oxidizing bacteria

摘要：Aims This study investigated the microbial ecology of a biodesulfurization system inoculated with indigenous (rather than commercial) sulfur-oxidizing bacteria (SOB), aiming to assess their rapid enrichment feasibility and identify optimization strategies.Methods and results Pilot-scale Shell-Paques reactors were established to remove hydrogen sulfide from natural gas, using an inoculum of indigenous SOB. Microbial community dynamics were tracked via high-throughput sequencing, while ecological interactions were analyzed by combining co-occurrence network analysis and niche overlap indices. Indigenous SOB were successfully enriched to an abundance of similar to 30% within 13 days. Thioalkalimicrobium emerged as the dominant SOB genus, differing from strains typically employed in commercial technologies. A key finding was the production of bipyramidal elemental sulfur with a rhombic structure as the primary product. Both network and niche overlap analyses revealed complex ecological interactions, indicating potential competition and mutualism between Thioalkalimicrobium and other dominant genera.Conclusions Indigenous SOB can be rapidly enriched within two weeks to achieve effective desulfurization and sulfur recovery. The ecological insights lay the groundwork for optimizing enrichment through targeted microbiota management.