文献类型：期刊文献

英文题名：Engineering the metabolism and morphology of the filamentous fungus Trichoderma reesei for efficient L-malic acid production

作者：Chen, Yumeng[1,2];Wang, Jiajia[1,2];Wang, Meng[1];Han, Ao[1];Zhao, Xinqing[3];Wang, Wei[1,2];Wei, Dongzhi[1,2]

机构：[1]State Key Lab Bioreactor Engn, 130 Meilong Rd, Shanghai, Peoples R China;[2]East China Univ Sci & Technol, Shanghai 200237, Peoples R China;[3]Shanghai Jiao Tong Univ, Shanghai, Peoples R China

年份：2023

卷号：387

外文期刊名：BIORESOURCE TECHNOLOGY

收录：;EI(收录号：20233414614782);WOS:【SCI-EXPANDED(收录号:WOS:001059680200001)】；

基金：This research was funded by the Natural Science Foundation of Shanghai (22ZR1417600 and 23ZR1414800) .

语种：英文

外文关键词：L-malic acid; C4-dicarboxylate transporter; Trichoderma reesei; Intrinsic pathway; Fungal morphology

摘要：L-malic acid (MA) is a vital platform chemical with huge market demand because of its broad industrial applications. A cell factory for MA production was engineered by strengthening the intrinsic pathway without inserting foreign genes into Trichoderma reesei. The native MA transporter gene in the T. reesei genome was characterized (trmae1), and its overexpression significantly improved MA production, which increased from 2 to 56.24 g/L. Native pyruvate carboxylase, malate dehydrogenase, malic enzyme, and glucose transporter were overexpressed further to improve the titer and yield of MA production. Fungal morphology was adapted to produce MA in the fermenter by deleting gul1. A titer of 235.8 g/L MA was produced from the final engineered strain in a 5-L fermenter with a yield of 1.48 mol of MA per mol of glucose and productivity of 1.23 g/L/h. This study provides novel insights for understanding and remodeling the MA synthesis pathway.