文献类型：期刊文献

英文题名：Microwave-assisted degradation of β-D-glucan from Ganoderma lucidum and the structural and immunoregulatory properties of oligosaccharide fractions

作者：Qin, Xiu[1,2];Ma, Guanhua[1];Liu, Liping[1];Feng, Jie[1];Zhou, Shuai[1];Han, Wei[2];Zhou, Jing[1,3];Liu, Yanfang[1];Zhang, Jingsong[1]

机构：[1]Natl Engn Res Ctr Edible Fungi, Shanghai Acad Agr Sci, Inst Edible Fungi, Key Lab Edible Fungi Resources & Utilizat South,Mi, Shanghai 201403, Peoples R China;[2]East China Univ Sci & Technol, Engn Res Ctr Pharmaceut Proc Chem, Sch Pharm, Shanghai Key Lab New Drug Design,Minist Educ, Shanghai 200237, Peoples R China;[3]Shanghai Baixin Biotech Co Ltd, Shanghai 201403, Peoples R China

年份：2022

卷号：220

起止页码：1197

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20242616368949);WOS:【SCI-EXPANDED(收录号:WOS:000861238600005)】；

基金：Acknowledgements This work was financially supported by Natural Science Foundation of Shanghai, China (Grant No. 20ZR1418700) , the Earmarked Fund for China Agriculture Research System, China (Grant No. CARS-20) , Lead- ing Talents Fund in Minhang District of Shanghai, China (Grant No. 201844) and SAAS Program for Excellent Research Team (Grant No. G2022003) .

语种：英文

外文关键词：beta-Glucooligosaccharide; HPAEC-PAD-MS/MS; Dectin-1

摘要：Microwave-assisted degradation of beta-(1 -> 3,1 -> 6)-D-glucan from Ganoderma lucidum and correlated immunoregulatory activities were investigated in this study. The optimal temperature and degradation time for microwave hydrothermal hydrolysis were 140 degrees C and 40 min, respectively. Under these conditions, a high yield of degradation rate (98.4 %) and abundant beta-oligosaccharide products (GLOS) with different degrees of polymerization (DP 2-24) were obtained. Four fractions including F1 (DP 2-8), F2 (DP 6-19), F3 (DP 8-24) and F4 (high DPs) with different average ratios of beta-(1 -> 3) to beta-(1 -> 6)-linked glucose units were isolated from GLOS. The structures of oligosaccharides with DP (2-6) in Fl were identified as linear beta-(1 -> 3)-linked glucooligosaccharides without or with beta-(1 -> 6)-linked glucose residues based on MS/MS analysis. The immunoregulation activity of beta-glucooligosaccharides was correlated with their DPs and the average ratios of beta-(1 -> 3) to beta-(1 -> 6)- linked glucose units. F4 fraction with high DPs and ratio of 3.29:1 exhibited higher immunoenhancing activity on inducing NF-kappa B activation through binding to dectin-1. Surface plasmon resonance (SPR) analysis indicated that beta-glucooligosaccharides could bind to Dectin-1 directly and the binding affinity increased with the increase of DPs and the ratios of beta-(1 -> 3)-linked glucose.