文献类型：期刊文献

英文题名：Difunctional Fluorescence Nanoparticles for Accurate Tracing of Nanopesticide Fate and Crop Protection Prepared by Flash Nanoprecipitation

作者：Chen, Kai[1,2];Wang, Yan[1];Cui, Haixin[1];Wei, Zhong[3];Jia, Xin[3];Liu, Zhiyong[3];Guo, Xuhong[2,3]

机构：[1]Chinese Acad Agr Sci, Beijing, Peoples R China;[2]East China Univ Sci & Technol, Shanghai, Peoples R China;[3]Shihezi Univ, Shihezi, Peoples R China

年份：2020

卷号：68

期号：3

起止页码：735

外文期刊名：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY

收录：;EI(收录号：20201008253018);WOS:【SCI-EXPANDED(收录号:WOS:000509427900005)】；

基金：We thank the financial support by the National Natural Science Foundation of China Grants (31701825, 51773061, and 51761135128), the National Key R&D Program of China (2016YFD0200500), and Key Scientific and Technological Project of Xinjiang Bingtuan (2018AB025).

语种：英文

外文关键词：fluorescence tracing; difunctional nanoparticles; pesticide delivery; flash nanoprecipitation; chlorophyll autofluorescence; insecticidal activity

摘要：Facile fabrication of difunctional nanoparticles (NPs) for pesticide delivery and imaging is still a fascinating challenge. Here, water-dispersible difunctional NPs were developed using flash nanoprecipitation (FNP) where self-assembling amphiphilic block copolymers were used to encapsulate a highly hydrophobic model pesticide, Lambda-cyhalothrin, and the fluorescent dye Nile red. The particle size (ranging from 158 to 280 nm) and fluorescence property of NPs could be controlled by varying the flow rate or Nile red feed concentration. The aggregation state and rearrangement of the dye molecules in the NPs were also investigated. IVIS imaging and confocal laser scanning microscopy analysis demonstrated that the resulting difunctional nanopesticide particles could allow accurate in situ tracking of the pesticide on the leaf surface, while effectively avoiding interference from chlorophyll autofluorescence. The difunctional NP suspension maintained high insecticidal activity and stability. This work demonstrates the feasibility and great potential of the FNP method in universal fabrication of multifunctional NPs with in situ pesticide tracing and crop protection capabilities.