文献类型：期刊文献

英文题名：Variants of lipopeptides produced by Bacillus licheniformis HSN221 in different medium components evaluated by a rapid method ESI-MS

作者：Li, Yi-Ming;Haddad, Namir I. A.;Yang, Shi-Zhong;Mu, Bo-Zhong[1]

机构：[1]E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China; E China Univ Sci & Technol, Inst Appl Chem, Shanghai 200237, Peoples R China

年份：2008

卷号：14

期号：3

起止页码：229

外文期刊名：INTERNATIONAL JOURNAL OF PEPTIDE RESEARCH AND THERAPEUTICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000258840200006)】；

基金：This work was supported by the National Natural Science Foundation of China (No. 50574040) and the grant from Shanghai Science and Technology Commission (No. 071607014).

语种：英文

外文关键词：lipopeptide; Bacillus licheniformis; medium components; ESI-MS

摘要：A lipopeptide producing strain was isolated from an oil field and identified as Bacillus licheniformis HSN221. Nine different substrates were used to cultivate the strain under the same incubation conditions. Using a rapid method, Electrospray Ionization Mass Spectrometry (ESI-MS) combined with Thin Layer Chromatography (TLC), nine different lipopeptide homologues were found and identified. The strain produced four [Leu]surfactin homologues, surfactin C13, surfactin C14, surfactin C15 and surfactin C16, when cultivated in the medium with glucose, yeast extract and ammonium chloride, but it produced five lichenysin homologues, lichenysin C12, lichenysin C13, lichenysin C14, lichenysin C15 and lichenysin C16, when cultivated in the remaining eight media. Additionally, it showed that the type and relative content of each homologue were consistent with in each medium which is helpful for optimizing the medium components to cultivate the similar species.