文献类型：期刊文献

英文题名：The relationship between features enabling SDHI fungicide binding to the Sc-Sdh complex and its inhibitory activity against Sclerotinia sclerotiorum

作者：Gao, Yangyang[1,2];He, Lifei[1,2];Zhu, Jiamei[1,2];Cheng, Jiagao[3];Li, Beixing[1,2];Liu, Feng[1,2];Mu, Wei[1,2]

机构：[1]Shandong Agr Univ, Shandong Prov Key Lab Biol Vegetable Dis & Insect, Tai An, Shandong, Peoples R China;[2]Shandong Agr Univ, Coll Plant Protect, 61 Daizong St, Tai An 271018, Shandong, Peoples R China;[3]East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab Chem Biol, Shanghai, Peoples R China

年份：2020

卷号：76

期号：8

起止页码：2799

外文期刊名：PEST MANAGEMENT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000534575000001)】；

基金：This research was supported by the National Key R&D Program of China (2016YFD0200500) and the Provincial Key Research and Development Program of Shandong (2017CXGC0207).

语种：英文

外文关键词：Sclerotinia sclerotiorum; SDHI fungicides; inhibitory activity; binding features; binding energy

摘要：BACKGROUND A new generation of succinate dehydrogenase inhibitors (SDHIs) with high efficiency and broad-spectrum antifungal activity has been frequently used in crop production. Sclerotinia stem rot is a major disease of various plants and crops caused by Sclerotinia sclerotiorum. Although benzovindiflupyr and isopyrazam reportedly have high activity against S. sclerotiorum, little is known about the bioactivity of different SDHIs classes against S. sclerotiorum or the mechanism of their differential antifungal activity. RESULTS The in vitro tests revealed that the pyrazole-4-carboxamides of SDHIs (benzovindiflupyr, isopyrazam, fluxapyroxad, pydiflumetofen) had the highest activity against S. sclerotiorum followed by pyridine carboxamides (boscalid), pyridinyl-ethyl benzamides (fluopyram) and thiazole carboxamides (thifluzamide), and of these thifluzamide showed poor antifungal activity with EC50 values greater than 6.01 mg L-1. The pyrazole-4-carboxamides of SDHIs showed satisfactory protective and curative activity against Sclerotinia stem rot. After treatment with the pyrazole-4-carboxamides of SDHIs, mitochondrial function in S. sclerotiorum decreased significantly. The enzyme activity assays revealed a lower affinity between thifluzamide and the Sc-Sdh complex than was observed for the other six fungicides, with IC50 values ranging from 0.0036 to 1.2088 mu mol L-1. Additionally, the docking positions of fungicides were similar, yet binding energies were different in the docking study with the Sdh complex. The correspondingly weaker hydrogen bonds may be responsible for the poor activity of thifluzamide against S. sclerotiorum. CONCLUSION Understanding different binding features of various SDHIs classes with the Sc-Sdh complex might be beneficial for the design and development of highly effective broad-spectrum fungicides to ensure high yield and quality in crops by reducing fungicide use.