文献类型：期刊文献

英文题名：MviN mediates the regulation of environmental osmotic pressure on esrB to control the virulence in the marine fish pathogen Edwardsiella piscicida

作者：Yin, Kaiyu[1];Zhang, Jin[1];Ma, Jiabao[1];Jin, Peng[1];Ma, Yue[1,2,3];Zhang, Yuanxing[1,2,3];Liu, Xiaohong[1,2,3];Wang, Qiyao[1,2,3]

机构：[1]East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China;[2]Shanghai Collaborat Innovat Ctr Biomfg Technol, 130 Meilong Rd, Shanghai 200237, Peoples R China;[3]Shanghai Engn Res Ctr Maricultured Anim Vaccines, Shanghai 200237, Peoples R China

年份：2020

卷号：239

外文期刊名：MICROBIOLOGICAL RESEARCH

收录：;EI(收录号：20202708889873);WOS:【SCI-EXPANDED(收录号:WOS:000565563300019)】；

基金：This work was sponsored by China Postdoctoral Science Foundation (2019M651420) and Shanghai Sailing Program (19YF1411500), as well as the National Key Research and Development Program of China (2018YFD0900500), the Ministry of Agriculture of China (CARS-47), and the Science and Technology Commission of Shandong and Shanghai Municipality (2017CXGC0103 and 17391902000).

语种：英文

外文关键词：Edwardsiella piscicida; EsrB; Lipid II flippase; Osmotic pressure; In vivo colonization

摘要：Edwardsiella piscicida is a notorious pathogen infecting diverse kinds of fish and causes substantial economic losses in the global aquaculture industries. The EsrA-EsrB two-component system plays a critical role in the regulation of virulence genes expression, including type III and type VI secretion systems (T3/T6SSs). In this study, the putative regulators of esrB were screened by the transposon insertion sequencing (TIS) technology. As a result, MviN, a lipid II flippase, was identified as a modulator to upregulate esrB and downstream T3/T6SS gene expression in the earlier growth phases while downregulate esrB at the later stages. Complement or overexpression of the mviN restored the esrB as well as T3/T6SS expression in the Delta mviN mutant strain. Moreover, MviN also mediated the regulation of environmental osmotic pressure on the expression of esrB. MviN was also found to significantly influence the in vivo colonization of E. piscicida in turbot. Collectively, this study enhanced our understanding of pathogenesis and virulence regulatory network of E. piscicida.