文献类型：期刊文献

英文题名：The biotoxicity of hydroxyapatite nanoparticles to the plant growth

作者：Jiang, Hao[1];Liu, Jin-Ku[1];Wang, Jian-Dong[1];Lu, Yi[1];Zhang, Min[1];Yang, Xiao-Hong[2];Hong, Dan-Jing[1]

机构：[1]E China Univ Sci & Technol, Key Lab Adv Mat, Shanghai 200237, Peoples R China;[2]Chizhou Univ, Dept Chem, Chizhou 247000, Peoples R China

年份：2014

卷号：270

起止页码：71

外文期刊名：JOURNAL OF HAZARDOUS MATERIALS

收录：;EI(收录号：20140917380817);WOS:【SCI-EXPANDED(收录号:WOS:000335109200009)】；

基金：This work was supported by the National Natural Science Foundation of China (Grant 21341007 and 21071024), Fundamental Research Funds for the Central Universities (Grant 222201313005) and State Key Laboratory of Pollution Control and Resource Reuse Foundation (Grant PCRRF13019).

语种：英文

外文关键词：Hydroxyapatite; Cytotoxicity; Hydrothermal method; Biotoxicity

摘要：In the present study, hydroxyapatite (HAP) nanoparticles of different particle sizes with high crystallinity and similiar structure were prepared by hydrothermal method. The crystal structure and particle size were characterized by X-ray diffraction pattern (XRD), transmission electron microscopy (TEM) and Fourier transform infrared (FT-IR) spectroscopy. Mung bean sprouts were first used as experimental models. Instead of by MIT assay, the cytoxicity of HAP nanoparticles were proved and evaluated by measuring the hypocotyle length of mung bean sprouts in the culture media. The result showed that the inhibition effect to the growth of mung bean sprouts enhanced when HAP nanoparticles existed. Culture media of HAP nanoparticles with different concentrations and particle sizes was prepared to investigate the level of inhibition effect to the growth of mung bean sprouts. The result found that hypocotyl length of mung bean sprouts were the shortest cultured in 5 mg/mL culture media in which the HAP nanoparticles were prepared by hydrothermal method for 24 h. It was concluded the inhibition effect depended on the amount of intracellular HAP nanoparticles. The nanostructure and Ca2+ concentration were considered as the main factors to cause cell apoptosis which was the reason of inhibition. The study provided a preliminary perspective about biotoxicity of HAP nanomaterials to the plant growth. (C) 2014 Elsevier B.V. All rights reserved.